Pathogenic Assessment of SfMNPV-Based Biopesticide on Spodoptera frugiperda (Lepidoptera: Noctuidae) Developing on Transgenic Soybean Expressing Cry1Ac Insecticidal Protein
| Autor: | Oderlei Bernardi, José Gomes da Silva Filho, Holly J. R. Popham, Paula Marçon, Matheus Rakes, Ana Paula Schneid Afonso da Rosa, Marcelo R Zakseski, Daniel Bernardi, Juliano de Bastos Pazini |
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| Rok vydání: | 2021 |
| Předmět: |
Insecticides
Veterinary medicine Baculoviridae viruses Population Spodoptera Zea mays Lepidoptera genitalia Hemolysin Proteins Bacterial Proteins parasitic diseases Animals education education.field_of_study Ecology biology PRAGAS DE PLANTAS fungi food and beverages General Medicine Plants Genetically Modified biology.organism_classification Nucleopolyhedroviruses Endotoxins Biopesticide Biological Control Agents Cry1Ac Larva Insect Science Soybean Proteins Noctuidae Fall armyworm Soybeans |
| Zdroj: | Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP |
| ISSN: | 1938-291X 0022-0493 |
| Popis: | Pathogenic assessment of a baculovirus-based biopesticide containing Spodoptera frugiperda multiple nucleopolyhedrovirus (SfMNPV: Baculoviridae: Alphabaculovirus) infecting fall armyworm, Spodoptera frugiperda (J. E. Smith, 1797) (Lepidoptera: Noctuidae) is reported. In the bioassays, neonates were infected with different doses of SfMNPV applied on Cry1Ac Bt soybean and non-Bt soybean. Our findings indicated that S. frugiperda neonates did not survive at 10 d post infection or develop into adults on Bt and non-Bt soybean sprayed with the field recommended dose of SfMNPV. In contrast, a proportion of the infected neonates developed into adults when infected with lower doses of SfMNPV (50%, 25%, and 10% of field dose) in both Bt and non-Bt soybean. However, S. frugiperda neonates surviving infection at the lowest virus doses on both soybean varieties showed longer neonate-to-pupa and neonate-to-adult periods, lower larval and pupal weights, reduced fecundity, and increased population suppression. Nevertheless, more pronounced pathogenicity of SfMNPV infecting neonates of S. frugiperda were verified on larvae that developed on Bt soybean. These findings revealed that, beyond mortality, the biopesticide containing SfMNPV also causes significant sublethal pathogenic effects on neonates of S. frugiperda developing on Bt and non-Bt soybean and suggested an additive effect among SfMNPV and Cry1Ac insecticidal protein expressed in Bt soybean. |
| Databáze: | OpenAIRE |
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