Lack of seronegative hepatitis C virus infections in patients with chronic renal failure
| Autor: | Laura E. Brannon, Kathleen Connor, Jennifer Everett-Kitchens, Enrique Martinez, Thomas C. Pearson, Frederick S. Nolte, Violet A. Kelley |
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| Rok vydání: | 2002 |
| Předmět: |
Male
Hepatitis C virus Hepacivirus Population medicine.disease_cause Serology Flaviviridae Humans Medicine Prospective Studies education Transplantation education.field_of_study biology Reverse Transcriptase Polymerase Chain Reaction business.industry Hepatitis C Antibodies Middle Aged biology.organism_classification medicine.disease Hepatitis C Immunology Kidney Failure Chronic RNA Viral Female Viral disease business Kidney disease |
| Zdroj: | Transplantation. 74:1473-1475 |
| ISSN: | 0041-1337 |
| DOI: | 10.1097/00007890-200211270-00022 |
| Popis: | Background. Recent reports have indicated that serologic testing for hepatitis C virus (HCV) in patients with chronic renal failure may be inadequate to detect infections in this patient population. Methods. We prospectively tested 258 patients with end-stage renal disease who were referred for evaluation for renal transplantation for anti-HCV using a second-generation enzyme immunoassay (EIA) and a second-generation qualitative reverse-transcriptase polymerase chain reaction (RT-PCR). We confirmed all positive EIAs with a third-generation recombinant immunoblot assay and genotyped RT-PCR-positive specimens. Results. We found that 22 patients (8.5%) had serological evidence of HCV infection. Nineteen (86%) of the antibody-positive patients were viremic (HCV RNA positive). All but 1 of the patients was infected with HCV genotype 1. None of the 233 HCV antibody-negative patients were shown to be viremic by RT-PCR. Conclusions. No additional HCV cases were detected by screening all patients for HCV RNA by RT-PCR. However, RT-PCR is a valuable adjunct to serology in antibody-positive patients to distinguish resolved from active infections. |
| Databáze: | OpenAIRE |
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