Faecalibacterium prausnitzii increases following fecal microbiota transplantation in recurrent Clostridioides difficile infection

Autor: Cecilia Magnusson, Olle Björkqvist, Torbjörn Norén, Ignacio Rangel, Jonas Halfvarson, Lena Serrander, Malin Bergman-Jungeström
Jazyk: angličtina
Rok vydání: 2021
Předmět:
Male
Molecular biology
Physiology
Faecalibacterium prausnitzii
Gut flora
Gastroenterology
Feces
Sequencing techniques
Recurrence
Antibiotics
Medicine and Health Sciences
Medicine
Homeostasis
DNA sequencing
Multidisciplinary
biology
Antimicrobials
Drugs
Genomics
Fecal Microbiota Transplantation
Middle Aged
Real-time polymerase chain reaction
Medical Microbiology
Female
Anatomy
Transcriptome Analysis
Research Article
DNA
Bacterial
Next-Generation Sequencing
Treatment response
medicine.medical_specialty
Clostridium Difficile
Science
Context (language use)
Microbial Genomics
Gastroenterology and Hepatology
Microbiology
Vancomycin
Internal medicine
Microbial Control
Genetics
Gastroenterologi
Humans
Aged
Pharmacology
Bacteria
business.industry
Gut Bacteria
Organisms
Biology and Life Sciences
Computational Biology
Fecal bacteriotherapy
biology.organism_classification
Genome Analysis
Gastrointestinal Microbiome
Research and analysis methods
Gastrointestinal Tract
Molecular biology techniques
Clostridium Infections
Microbiome
business
Physiological Processes
Digestive System
Clostridioides
Zdroj: PLoS ONE, Vol 16, Iss 4, p e0249861 (2021)
PLoS ONE
Popis: Objective Fecal microbiota transplantation (FMT) is a highly effective treatment for Clostridioides difficile infection (CDI). However, the fecal transplant’s causal components translating into clearance of the CDI are yet to be identified. The commensal bacteria Faecalibacterium prausnitzii may be of great interest in this context, since it is one of the most common species of the healthy gut microbiota and produces metabolites with anti-inflammatory properties. Although there is mounting evidence that F. prausnitzii is an important regulator of intestinal homeostasis, data about its role in CDI and FMT are relatively scarce. Methods Stool samples from patients with recurrent CDI were collected to investigate the relative abundance of F. prausnitzii before and after FMT. Twenty-one patients provided fecal samples before the FMT procedure, at 2 weeks post-FMT, and at 2–4 months post-FMT. The relative abundance of F. prausnitzii was determined using quantitative polymerase chain reaction. Results The abundance of F. prausnitzii was elevated in samples (N = 9) from donors compared to pre-FMT samples (N = 15) from patients (adjusted PF. prausnitzii between responders (N = 11) and non-responders (N = 4) was found before FMT (P = 0.85). In patients with CDI, the abundance of F. prausnitzii significantly increased in the 2 weeks post-FMT samples (N = 14) compared to the pre-FMT samples (N = 15, adjusted P Conclusions FMT increases the relative abundance of F. prausnitzii in patients with recurrent CDI, and this microbial shift remains several months later. The baseline abundance of F. prausnitzii in donors or recipients was not associated with future treatment response, although a true predictive capacity cannot be excluded because of the limited sample size. Further studies are needed to discern whether F. prausnitzii plays an active role in the resolution of CDI.
Databáze: OpenAIRE
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