Evaluation of a Novel System for Hypothermic Oxygenated Pulsatile Perfusion Preservation

Autor: M.C.J.M. Schreinemachers, Mario Sitzia, Sandrine Florquin, Alma Zernecke, Benedict M. Doorschodt, Wei Lai, Rene Tolba
Přispěvatelé: Amsterdam institute for Infection and Immunity, Pathology
Rok vydání: 2009
Předmět:
030213 general clinical medicine
Pathology
Adenosine
Time Factors
Swine
medicine.medical_treatment
Medicine (miscellaneous)
Kidney
Kidney Function Tests
0302 clinical medicine
Laser-Doppler Flowmetry
Insulin
Cold Ischemia
Equipment Design
General Medicine
Glutathione
Immunohistochemistry
Perfusion
Pulsatile Flow
Models
Animal
Tissue and Organ Harvesting
Female
medicine.medical_specialty
Allopurinol
Organ Preservation Solutions
Biomedical Engineering
Cold storage
030209 endocrinology & metabolism
Bioengineering
Transplantation
Autologous
Renal Circulation
Microcirculation
Biomaterials
03 medical and health sciences
Raffinose
medicine
Animals
Viaspan
Kidney surgery
Cell Proliferation
Machine perfusion
business.industry
Kidney metabolism
Recovery of Function
Kidney Transplantation
Autotransplantation
Oxygen
Transplantation
business
Zdroj: International journal of artificial organs, 32(10), 728-738. Wichtig Publishing
ISSN: 1724-6040
0391-3988
Popis: Background Recently, a novel innovative machine perfusion (MP) system for hypothermic oxygenated pulsatile perfusion called the Airdrive (AD) has been developed. The aim of the study was to evaluate the biological safety of the AD system for perfusion preservation of kidney grafts in a porcine autotransplantation model using the low-viscosity perfusion solution Polysol (PS) in comparison with cold storage (CS) using PS or the University of Wisconsin solution (UW). In addition, we evaluated real-time microcirculation parameters. At sacrifice, grafts were retrieved for histological analysis and immunohistochemistry Methods After assessment of the microcirculation, left kidneys were retrieved. Following the washout, kidneys were preserved for 20 hr using AD-PS, CS-PS or CS-UW. Thereafter, contralateral kidneys were removed followed by heterotopic autotransplantation of the preserved graft. Seven days after transplantation animals were sacrificed with retrieval of the grafts for histological analysis. Renal function, renal microcirculation and tissue injury including the proliferative response of tubular epithelial cells (TECs) were compared. Results Preservation using AD-PS or CS-PS resulted in higher microcirculatory flow compared with CS-UW. Improved recovery of renal function was seen in the AD-PS and CS-PS groups compared with CS-UW. Structural integrity was better preserved using AD-PS compared with both CS groups. Proliferative response of TECs was higher in CS-UW preserved grafts compared to grafts preserved using AD-PS. Conclusion This study demonstrates the biological safety of the AD system in a porcine autotransplantation model. Also, the microcirculation was better preserved and less morphological injury was observed after 20 hr MP compared with CS.
Databáze: OpenAIRE
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