A Novel Laser-Based Zebrafish Model for Studying Traumatic Brain Injury and Its Molecular Targets

Autor: Maria A. Tikhonova, Nikolai A. Maslov, Alim A. Bashirzade, Eugenyi V. Nehoroshev, Vladislav Y. Babchenko, Nadezhda D. Chizhova, Elena O. Tsibulskaya, Anna A. Akopyan, Evgeniya V. Markova, Yi-Ling Yang, Kwok-Tung Lu, Allan V. Kalueff, Lyubomir I. Aftanas, Tamara G. Amstislavskaya
Jazyk: angličtina
Rok vydání: 2022
Předmět:
NEUROMORPHOLOGY
ZEBRA FISH
PROTEIN EXPRESSION
Pharmaceutical Science
CONVALESCENCE
ANIMAL MODEL
CELL DAMAGE
UNCLASSIFIED DRUG
NERVOUS SYSTEM INFLAMMATION
ANIMAL EXPERIMENT
NEUROINFLAMMATION
CONSTRUCT VALIDITY
MICROGLIA
ANXIETY DISORDER
ADULT
TRAUMATIC BRAIN INJURY
ANIMAL TISSUE
NONHUMAN
BRAIN DERIVED NEUROTROPHIC FACTOR
CELL ACTIVATION
ARTICLE
HYPOLOCOMOTION
traumatic brain injury
laser
animal model
zebrafish
neurodegeneration
neurorepair
neuroinflammation
behavior
TELENCEPHALON
MALE
ZEBRAFISH
HYDROCORTISONE
IONIZED CALCIUM BINDING ADAPTER MOLECULE 1 PROTEIN
LOCOMOTION
NEURODEGENERATION
NEUROREPAIR
BEHAVIOR ASSESSMENT
VELOCITY
HYPOXIA INDUCIBLE FACTOR 1ALPHA
FEMALE
CONTROLLED STUDY
ANIMAL CELL
CELL PROTEIN
MORPHOLOGY
LASER
NEURON SPECIFIC NUCLEAR PROTEIN
NOVEL TANK TEST
MOLECULARLY TARGETED THERAPY
FACE VALIDITY
BEHAVIOR
Zdroj: Pharmaceutics
Pharmaceutics; Volume 14; Issue 8; Pages: 1751
DOI: 10.3390/pharmaceutics14081751&partnerID=40&md5=bda19df98ae2372fe6a731d67450bbcc
Popis: Traumatic brain injury (TBI) is a major public health problem. Here, we developed a novel model of non-invasive TBI induced by laser irradiation in the telencephalon of adult zebrafish (Danio rerio) and assessed their behavior and neuromorphology to validate the model and evaluate potential targets for neuroreparative treatment. Overall, TBI induced hypolocomotion and anxiety-like behavior in the novel tank test, strikingly recapitulating responses in mammalian TBI models, hence supporting the face validity of our model. NeuN-positive cell staining was markedly reduced one day, but not seven days, after TBI, suggesting increased neuronal damage immediately after the injury, and its fast recovery. The brain-derived neurotrophic factor (Bdnf) level in the brain dropped immediately after the trauma, but fully recovered seven days later. A marker of microglial activation, Iba1, was elevated in the TBI brain, albeit decreasing from Day 3. The levels of hypoxia-inducible factor 1-alpha (Hif1a) increased 30 min after the injury, and recovered by Day 7, further supporting the construct validity of the model. Collectively, these findings suggest that our model of laser-induced brain injury in zebrafish reproduces mild TBI and can be a useful tool for TBI research and preclinical neuroprotective drug screening. © 2022 by the authors. Saint Petersburg State University, SPbU: 73026081; Russian Science Foundation, RSF: 20-65-46006 We thank Alisa S. Belova for technical support in experimental manipulations and cortisol assay. We also thank Anatoly A. Maslov for the idea of using laser radiation to introduce brain damage. A.V.K. lab is supported by St. Peterburg State University funds (Pure ID 73026081). This study was funded by Russian Science Foundation (grant No. 20-65-46006).
Databáze: OpenAIRE
Externí odkaz: