Next-generation protein analysis in the pathology department
| Autor: | Amélie Dendooven, Glenn Broeckx, Amaryllis H. Van Craenenbroeck, Karin Schildermans, Melek Ahmed, Geert Baggerman, Patrick Pauwels |
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| Rok vydání: | 2019 |
| Předmět: |
Proteomics
0301 basic medicine Pathology medicine.medical_specialty Computer science Mass spectrometry imaging Workflow Pathology and Forensic Medicine Imaging modalities 03 medical and health sciences 0302 clinical medicine Predictive Value of Tests medicine Humans Pathology Clinical Reproducibility of Results Protein level Pathology Department Hospital General Medicine Immunohistochemistry High-Throughput Screening Assays 030104 developmental biology Mrna level Spectrometry Mass Matrix-Assisted Laser Desorption-Ionization 030220 oncology & carcinogenesis Human medicine Diffusion of Innovation |
| Zdroj: | Journal of clinical pathology |
| ISSN: | 1472-4146 0021-9746 |
| DOI: | 10.1136/jclinpath-2019-205864 |
| Popis: | Traditionally, immunohistochemistry (IHC) is used by pathologists to localise specific proteins or peptides in tissue slides. In the era of personalised medicine, however, molecular tissue analysis becomes indispensable for correct diagnosis, prognosis and therapeutic decision, not only on the DNA or mRNA level but also on the protein level. Combining molecular information with imaging presents many advantages. Therefore, matrix-assisted laser desorption/ionisation imaging mass spectrometry (MALDI IMS) is a promising technique to be added to the armamentarium of the pathologist. Here, we focus on the workflow, advantages and drawbacks of both MALDI IMS and IHC. We also briefly discuss a few other protein imaging modalities and give examples of applications. |
| Databáze: | OpenAIRE |
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