Characterization of interleukin-1β mRNA expression in chicken macrophages in response to avian reovirus
Autor: | Yung Fu Wu, Jui Huang Shien, Shiow-Her Chiou, Hung-Jen Liu, Long Huw Lee |
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Rok vydání: | 2008 |
Předmět: |
Orthoreovirus
Avian Aziridines Interleukin-1beta Ammonium Chloride Virus Virology Protein biosynthesis Animals RNA Messenger Orthoreovirus Cells Cultured RNA Double-Stranded Messenger RNA biology Macrophages Virion RNA biology.organism_classification Molecular biology Reoviridae Infections Reverse transcription polymerase chain reaction RNA silencing Viral replication RNA Viral Chickens |
Zdroj: | Journal of General Virology. 89:1059-1068 |
ISSN: | 1465-2099 0022-1317 |
DOI: | 10.1099/vir.0.82957-0 |
Popis: | Inhibitors of viral disassembly or RNA and protein synthesis, viral disassembly intermediates (infectious subviral particles, ISVP), binary ethylenimine-inactivated virions, and viral particles lacking genomic double-stranded (ds) RNA (empty particles) were used to assess the expression of interleukin-1β (IL-1β) mRNA in chicken (chIL-1β) macrophages in response to avian reovirus. The results demonstrate that two distinct expression patterns of chIL-1β mRNA mediated by different steps in viral replication were found. Viral disassembly was required for the induction of a rapid, transient expression pattern of chIL-1β mRNA that was rapidly induced at 30 min, with maximal levels reached by 2 h, and fell to a low level within 6 h post-inoculation, while viral RNA synthesis rather than protein translation, which was subsequent to membrane penetration, was required to induce a stable, sustained expression pattern of chIL-1β mRNA that occurred at and after 6 h post-inoculation. In addition, the induction of chIL-1β mRNA expression by the empty particles and ISVP was extremely weak, compared with the active dsRNA+ virions or binary ethylenimine-inactivated virions, suggesting that the presence of dsRNA, even if transcriptionally inactive, may be an important factor in this response. |
Databáze: | OpenAIRE |
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