A quantitative method for staging mouse embryos based on limb morphometry
| Autor: | Marco Musy, Joan T. Richtsmeier, Alexandre Robert-Moreno, Kevin Flaherty, Jelena Raspopovic, James Sharpe |
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| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Limb Buds Ontogeny Gestational Age 03 medical and health sciences Limb bud Mice Techniques and Resources Morphogenesis Animals Sonic hedgehog Molecular Biology Staging system Morphometrics biology Gene Expression Regulation Developmental Embryo Anatomy Embryo Mammalian Embryonic stem cell Hindlimb 030104 developmental biology biology.protein Limb morphogenesis Algorithms Developmental Biology |
| Popis: | To determine the developmental stage of embryonic mice we apply a geometric morphometric approach to the changing shape of the mouse limb bud as it grows from embryonic day 10 to embryonic day 15 post conception. As the ontogenetic sequence results in the de novo emergence of shape features not present in the early stages, we have created a standard ontogenetic trajectory for limb bud development – a quantitative characterization of shape change during limb morphogenesis. This trajectory of form as a function of time also gives us the reverse function: the ability to infer developmental stage from form, with a typical uncertainty of 2 hours. We introduce eMOSS (embryonic Mouse Ontogenetic Staging System, freely available at limbstaging.embl.es) as a fast, reliable and convenient on-line tool for staging embryos from 2D images of their limb buds, and illustrate its use in phenotyping early limb abnormalities. |
| Databáze: | OpenAIRE |
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