Dynamic Observation of Oxygenation-Induced Contraction of and Transient Fiber-Network Formation–Disassembly in Cultured Human Brain Microvascular Endothelial Cells
| Autor: | Hidetaka Takeda, Masahiro Kobari, Kouji Inoue, Minoru Tomita, Masaki Takao, Masako Yokoyama, Yasuo Fukuuchi, Norio Tanahashi |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
Contraction (grammar)
Cell Fiber network Cell morphology 030218 nuclear medicine & medical imaging 03 medical and health sciences 0302 clinical medicine Cell Movement medicine Humans Microscopy Phase-Contrast Cytoskeleton Cells Cultured Cell Size Chemistry Microcirculation Brain Human brain Anatomy Oxygenation Oxygen medicine.anatomical_structure Membrane Neurology Cerebrovascular Circulation Biophysics Endothelium Vascular Neurology (clinical) Cardiology and Cardiovascular Medicine 030217 neurology & neurosurgery |
| Zdroj: | Journal of Cerebral Blood Flow & Metabolism. 23:821-828 |
| ISSN: | 1559-7016 0271-678X |
| Popis: | Oxygenation-induced contraction of nonconfluent cultured human brain microvascular endothelial cells (HBECs, n = 30) was examined by video-enhanced contrast-differential interferential contrast microscopy. After administering a continuous gentle blow of pure oxygen gas to the surface of the medium just above the flattened HBEC, the plasma membrane exhibited tensioning and wrinkling, resulting in a strong contraction of the cell body by 14 ± 7% ( P < 0.001). When the cell stopped contracting, transient formation of a fiber network starting from certain spots (possibly adhesion plaques, though these were not visible in the majority of cases) and expanding to the whole cell was observed. The occurrence of fiber network formation was statistically significant (26 of 30 separate cells, P < 0.05). After cessation of oxygen delivery, the observed network of fibers broke up rapidly (in a period of 3.3 ± 1.2 seconds) into small particles of |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|