Chemiluminescent Optical Fiber Immunosensor Combining Surface Modification and Signal Amplification for Ultrasensitive Determination of Hepatitis B Antigen
| Autor: | Rongbin Nie, Jingwen Huang, Li Yang, Xuexue Xu |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
HBsAg
Hepatitis B virus Optical fiber Letter Biotin 02 engineering and technology lcsh:Chemical technology 01 natural sciences Biochemistry optical fiber immunosensor Analytical Chemistry law.invention Antigen law medicine Humans lcsh:TP1-1185 Fiber Hepatitis B e Antigens Electrical and Electronic Engineering Instrumentation Optical Fibers Chemiluminescence Immunoassay Chromatography Hepatitis B Surface Antigens hepatitis B e antigen Chemistry 010401 analytical chemistry virus diseases Hepatitis B 021001 nanoscience & nanotechnology medicine.disease Atomic and Molecular Physics and Optics digestive system diseases 0104 chemical sciences signal amplification hepatitis B surface antigen HBeAg DNA Viral Surface modification 0210 nano-technology surface modification |
| Zdroj: | Sensors, Vol 20, Iss 4912, p 4912 (2020) Sensors (Basel, Switzerland) |
| ISSN: | 1424-8220 |
| Popis: | Optical fiber based immunosensors are very attractive for biomarker detection. In order to improve the sensor response, we propose a promising strategy which combines porous-layer modification of the fiber surface and streptavidin-biotin-peroxidase nano-complex signal amplification in chemiluminescent detection. Two hepatitis B antigens, hepatitis B surface antigen (HBsAg) and hepatitis B e antigen (HBeAg), are used as the targets for analysis using the proposed sensor. Comparing to immunoassays using normal optical fiber sensors, the response of the present sensor is enhanced by a factor of 4.8 and 6.7 for detection of HBsAg and HBeAg, respectively. The limit-of-quantitation of the proposed method is as low as 0.3 fg/mL (0.01 fg/mL) with a wide linear response range of 3 fg/mL–150 ng/mL (0.1 fg/mL–160 ng/mL) for sensing HBsAg (HBeAg). Quantitative determination of HBsAg and HBeAg in human serum samples is performed, showing the applicability of the proposed method for biomarker detection. |
| Databáze: | OpenAIRE |
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