Effects of allyl isothiocyanate on the expression, function, and its mechanism of ABCA1 and ABCG1 in pulmonary of COPD rats
| Autor: | Nian-Xia Sun, Chong-Yang Wang, Ting-Ting Dai, Dian-Lei Wang, Meng-Wen Wang, Wen-Tao Zhu, Fu-Lin Tao, Ya-Nan Zhao, Chen-Hui Li, Zhi-Li Han |
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| Rok vydání: | 2021 |
| Předmět: |
Male
Immunology Inflammation Pharmacology Cell Line Rats Sprague-Dawley Pulmonary Disease Chronic Obstructive chemistry.chemical_compound Isothiocyanates Smoke medicine Animals Immunology and Allergy Oil Red O Liver X receptor Lung ATP Binding Cassette Transporter Subfamily G Member 1 Liver X Receptors COPD biology Lipid metabolism Allyl isothiocyanate medicine.disease Rats Up-Regulation Gene Expression Regulation chemistry ABCA1 Food Preservatives biology.protein Tumor necrosis factor alpha medicine.symptom ATP Binding Cassette Transporter 1 |
| Zdroj: | International Immunopharmacology. 101:108373 |
| ISSN: | 1567-5769 |
| Popis: | BACKGROUND AND AIMS Allyl isothiocyanate(AITC) has been shown to play an important role in the improved symptoms of chronic obstructive pulmonary disease(COPD) and the inhibition of inflammation, but the role in COPD lipid metabolism disorder and the molecular mechanism remains unclear. We aimed to explore whether and how AITC affects COPD by regulating lipid metabolism and inflammatory response. METHODS The COPD rat model was established by cigarette smoke exposure. Cigarette smoke extract stimulated 16HBE cells to induce a cell model. The effect of AITC treatment was detected by lung function test, H&E staining, Oil red O staining, immunohistochemistry, ELISA, CCK-8, HPLC, fluorescence efflux test, siRNA, RT-PCR, and Western blotting. Biological analysis was performed to analyze the results. Graphpad Prism 8.0 software was used for statistical analysis. RESULTS AITC can improve lung function and pathological injury in COPD rats. The levels of IL-1 β and TNF- α in the AITC treatment group were significantly lower than those in the model group(P |
| Databáze: | OpenAIRE |
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