Effect of phosphorylated cyclocytidine on deoxyribonucleic acid polymerase
| Autor: | Kanai Tadashi, Kanzawa Fumihiko, Hoshi Akio, Ichino Motonobu, Kuretani Kazuo |
|---|---|
| Rok vydání: | 1973 |
| Předmět: |
Male
DNA polymerase Aracytidine Antineoplastic Agents Cytosine Nucleotides Tritium Biochemistry Anhydrides Mice chemistry.chemical_compound Animals Carbon Radioisotopes Leukemia L1210 Pharmacology chemistry.chemical_classification Leukemia Experimental biology Phosphotransferases Cyclocytidine Cytarabine Deoxyribonucleic acid polymerase Nucleosides DNA Neoplasm Molecular biology Enzyme chemistry DNA Nucleotidyltransferases biology.protein Phosphorylation Deoxycytidine DNA Thymidine |
| Zdroj: | Biochemical Pharmacology. 22:2829-2834 |
| ISSN: | 0006-2952 |
| Popis: | Since cyclocytidine primarily inhibits the incorporation ofthymidine into DNA of cultured cells, the inhibition of DNA polymerase by some cyclocytidine derivatives was examined. Cyclocytidine 5'-monophosphate (cycloCMP) and aracytidine 5'-monophosphate (araCMP) markedly inhibited DNA polymerase, whereas cyclocytidine and aracytidine did not inhibit the enzyme in cell-free systems. Inhibitory potency of cyclo-CMP was about 1/15th that of araCMP. The possibility of phosphorylation of cyclo-cytidine to cyclo CMP was also examined, and it was revealed that cyclocytidine was phosphorylated at a rate 1/7th that of aracytidine or 1/10th that of deoxycytidine. |
| Databáze: | OpenAIRE |
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