Stromal Cells Covering Omental Fat-Associated Lymphoid Clusters Trigger Formation of Neutrophil Aggregates to Capture Peritoneal Contaminants
| Autor: | Beth E. P. Henderson, Mark Nixon, Cécile Bénézech, Pete Smith, Lucy H. Jackson-Jones, Marlene Magalhaes, Neil C. Henderson, Ross Dobie, Sonja Vermeren, Matthieu Vermeren, Katie J. Mylonas, Jordan R. Portman, Damian J. Mole, Laura Denby |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Stromal cell Neutrophils Chemokine CXCL1 Immunology Peritonitis Adipose tissue Gene Expression Cell Communication Biology Extracellular Traps Epithelium Microbiology Tissue Culture Techniques 03 medical and health sciences Mice 0302 clinical medicine Protein-Arginine Deiminase Type 4 medicine Escherichia coli Immunology and Allergy Animals Humans Lymphocytes Neutrophil aggregation Mice Knockout Sequence Analysis RNA Zymosan Epithelial Cells Neutrophil extracellular traps medicine.disease Appendicitis CXCL1 Mesothelium Mice Inbred C57BL 030104 developmental biology Infectious Diseases medicine.anatomical_structure Neutrophil Infiltration 030220 oncology & carcinogenesis Acute Disease Female Single-Cell Analysis Stromal Cells Omentum Mesothelial Cell |
| Zdroj: | Jackson-Jones, L H, Smith, P, Portman, J R, Magalhaes, M S, Mylonas, K J, Vermeren, M M, Nixon, M, Henderson, B E P, Dobie, R, Vermeren, S, Denby, L, Henderson, N C, Mole, D J & Bénézech, C 2020, ' Stromal Cells Covering Omental Fat-Associated Lymphoid Clusters Trigger Formation of Neutrophil Aggregates to Capture Peritoneal Contaminants ', Immunity, vol. 52, no. 4, pp. 700-715.e6 . https://doi.org/10.1016/j.immuni.2020.03.011 |
| ISSN: | 1097-4180 |
| DOI: | 10.1016/j.immuni.2020.03.011 |
| Popis: | The omentum is a visceral adipose tissue rich in fat-associated lymphoid clusters (FALCs) that collects peritoneal contaminants and provides a first layer of immunological defense within the abdomen. Here, we investigated the mechanisms that mediate the capture of peritoneal contaminants during peritonitis. Single-cell RNA sequencing and spatial analysis of omental stromal cells revealed that the surface of FALCs were covered by CXCL1+ mesothelial cells, which we termed FALC cover cells. Blockade of CXCL1 inhibited the recruitment and aggregation of neutrophils at FALCs during zymosan-induced peritonitis. Inhibition of protein arginine deiminase 4, an enzyme important for the release of neutrophil extracellular traps, abolished neutrophil aggregation and the capture of peritoneal contaminants by omental FALCs. Analysis of omental samples from patients with acute appendicitis confirmed neutrophil recruitment and bacterial capture at FALCs. Thus, specialized omental mesothelial cells coordinate the recruitment and aggregation of neutrophils to capture peritoneal contaminants. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|