Real-time optical detection of methicillin-resistant Staphylococcus aureus using lytic phage probes
| Autor: | April A. Krumnow, Iryna Sorokulova, Rajesh Guntupalli, Oleg Pustovyy, Vitaly Vodyanoy, Eric Olsen |
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| Rok vydání: | 2007 |
| Předmět: |
Microscopy
Staphylococcus aureus Meticillin biology Biomedical Engineering Biophysics Molecular Probe Techniques General Medicine Bacillus subtilis Biosensing Techniques biology.organism_classification medicine.disease_cause Langmuir–Blodgett film Methicillin-resistant Staphylococcus aureus Microbiology Bacteriophage Lytic cycle Salmonella enterica Electrochemistry medicine Methicillin Resistance Staphylococcus Phages Biotechnology medicine.drug |
| Zdroj: | Biosensorsbioelectronics. 24(1) |
| ISSN: | 0956-5663 |
| Popis: | Staphylococcus aureus ( S. aureus )-specific bacteriophage was used as a probe for detection of methicillin-resistant S. aureus (MRSA) in aqueous solution using a novel optical method. Biorecognition phage monolayers transferred to glass substrates using Langmuir–Blodgett (LB) technique were exposed individually to MRSA in solution at logarithmic concentrations ranging from 10 6 to 10 9 cfu/ml, and observed for real-time binding using a CytoViva™ optical light microscope system. Results indicate that LB monolayers possessed high levels of elasticity ( K ), measuring 22 and 29 mN/m for 10 9 and 10 11 pfu/ml phage concentrations, respectively. Near-instantaneous MRSA–phage binding produced 33 ± 5%, 10 ± 1%, 1.1 ± 0.1%, and 0.09 ± 0.01% coverage of the substrate that directly correlated to a decrease in MRSA concentrations of 10 9 , 10 8 , 10 7 , and 10 6 cfu/ml. The exclusive selectivity of phage monolayers was verified with Salmonella enterica subsp. enterica serovar typhimurium ( S. typhimurium ) and Bacillus subtilis . |
| Databáze: | OpenAIRE |
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