High Prevalence of CTX-M-15-Producing O25b-ST131Escherichia coliClone in Bulgarian Hospitals
Autor: | Dobrinka Ivanova, Ivan Mitov, M. Sredkova, Kalina Bojkova, Temenuga Stoeva, Adolf Bauernfeind, Emma Keuleyan, Raina Gergova, Boyka Markova, Ines Schneider, Rumyana Markovska |
---|---|
Rok vydání: | 2012 |
Předmět: |
Microbiology (medical)
clone (Java method) Immunology Microbial Sensitivity Tests Biology medicine.disease_cause Microbiology beta-Lactam Resistance beta-Lactamases Antibiotic resistance Phylogenetics Escherichia coli polycyclic compounds medicine Humans Typing Bulgaria Escherichia coli Infections Phylogeny Pharmacology High prevalence Phylogenetic tree biochemical phenomena metabolism and nutrition bacterial infections and mycoses Anti-Bacterial Agents Clone Cells Random Amplified Polymorphic DNA Technique RAPD Carbapenems Population Surveillance Isoelectric Focusing |
Zdroj: | Microbial Drug Resistance. 18:390-395 |
ISSN: | 1931-8448 1076-6294 |
Popis: | According to the European Antimicrobial Resistance Surveillance System project results, Bulgaria has become one of the European countries with dramatically increasing rates of extended-spectrum beta-lactamase (ESBL) producers. The aim of this work was to investigate the epidemiology of ESBL-producing Escherichia coli clinical isolates in Bulgaria, collected from seven clinical centers in three towns, during two study periods: 2002-2003 and 2006-2009. For 193 ESBL-producing E. coli isolates random amplified polymorphic DNA (RAPD) analyses, phylogenetic typing, and screening for O25b-ST131 isolates were carried out. Antimicrobial susceptibility, ESBL-type and transferability of resistance determinants were analyzed. Four different ESBL-types, namely TEM-139, SHV-12, CTX-M-3, and CTX-M-15 were found. CTX-M-15 dominated, being found in 88% of the isolates. RAPD-typing revealed 35 types, among which type A dominated, comprising 65% of the isolates. Sixty-eight percent of the 193 isolates belonged to the O25b-ST131 clone, to the phylogenetic group B2, mostly showed RAPD-type A (92%) and were found in all participating hospitals. O25b-ST131 isolates predominantly produced CTX-M-15 (96%), and less SHV-12 (n=3) or TEM-139 (n=2). In conclusion, this study demonstrated for the first time the country-wide dissemination of a highly resistant B2 O25b-ST131 CTX-M-15 producing E. coli clone in Bulgaria. |
Databáze: | OpenAIRE |
Externí odkaz: |