Origanum vulgare essential oil: antibacterial activities and synergistic effect with polymyxin B against multidrug-resistant Acinetobacter baumannii
| Autor: | Clóvis Moreira, Beatriz Bohns Pruski, Stella Buchhorn de Freitas, Daiane D. Hartwig, Suélen Cavalheiro Amaral, Antonio Sergio Varela Junior, Suzane Olachea Allend, Daniela Isabel Brayer Pereira, Marcos Roberto Alves Ferreira |
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| Rok vydání: | 2020 |
| Předmět: |
Acinetobacter baumannii
0301 basic medicine medicine.drug_class Antibiotics Gene Expression Microbial Sensitivity Tests beta-Lactamases Microbiology 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Antibiotic resistance Drug Resistance Multiple Bacterial Origanum Oils Volatile Genetics medicine Carvacrol Molecular Biology Thymol Polymyxin B Polycyclic Sesquiterpenes biology Drug Synergism General Medicine biology.organism_classification Antimicrobial Anti-Bacterial Agents Cephalosporins Drug Combinations Aminoglycosides 030104 developmental biology Carbapenems chemistry DNA Gyrase 030220 oncology & carcinogenesis Cymenes Fluoroquinolones medicine.drug |
| Zdroj: | Molecular Biology Reports. 47:9615-9625 |
| ISSN: | 1573-4978 0301-4851 |
| Popis: | Antimicrobial resistance is increasing around the world and the search for effective treatment options, such as new antibiotics and combination therapy is urgently needed. The present study evaluates oregano essential oil (OEO) antibacterial activities against reference and multidrug-resistant clinical isolates of Acinetobacter baumannii (Ab-MDR). Additionally, the combination of the OEO and polymyxin B was evaluated against Ab-MDR. Ten clinical isolates were characterized at the species level through multiplex polymerase chain reaction (PCR) for the gyrB and blaOXA-51-like genes. The isolates were resistant to at least four different classes of antimicrobial agents, namely, aminoglycosides, cephems, carbapenems, and fluoroquinolones. All isolates were metallo-β-lactamase (MβL) and carbapenemase producers. The major component of OEO was found to be carvacrol (71.0%) followed by β-caryophyllene (4.0%), γ-terpinene (4.5%), p-cymene (3,5%), and thymol (3.0%). OEO showed antibacterial effect against all Ab-MDR tested, with minimum inhibitory concentrations (MIC) ranging from 1.75 to 3.50 mg mL−1. Flow cytometry demonstrated that the OEO causes destabilization and rupture of the bacterial cell membrane resulting in apoptosis of A. baumannii cells (p |
| Databáze: | OpenAIRE |
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