Differential immunostimulating effect of Granulocyte-macrophage colony-stimulating factor (GM-CSF), Granulocyte colony-stimulating factor (G-CSF) and Interferon γ (IFNγ) after severe trauma
| Autor: | Sascha Flohé, Christian Waydhas, Sven Lendemans, F. U. Schade, E. Kreuzfelder |
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| Rok vydání: | 2007 |
| Předmět: |
Adult
Male Allergy Adolescent T-Lymphocytes Immunology Enzyme-Linked Immunosorbent Assay Monocytes Flow cytometry Sepsis Immunocompromised Host Interferon-gamma Adjuvants Immunologic Granulocyte Colony-Stimulating Factor medicine Humans Interferon gamma Prospective Studies Receptor Aged Aged 80 and over Pharmacology B-Lymphocytes medicine.diagnostic_test Tumor Necrosis Factor-alpha business.industry Granulocyte-Macrophage Colony-Stimulating Factor HLA-DR Antigens Middle Aged Flow Cytometry medicine.disease Interleukin-10 Granulocyte colony-stimulating factor Granulocyte macrophage colony-stimulating factor Severe trauma Receptors Granulocyte-Macrophage Colony-Stimulating Factor Cytokines Wounds and Injuries Female business medicine.drug |
| Zdroj: | Inflammation Research. 56:38-44 |
| ISSN: | 1420-908X 1023-3830 |
| Popis: | Severe trauma leads to an increased vulnerability to bacterial sepsis. In the present study, we compared the immunostimulating potential of granulocyte-colony stimulating-factor (G-CSF), granulocyte-macrophage colony-stimulating factor (GM-CSF) and interferon-gamma (IFN-gamma).Prospective clinical experimental study.University hospital intensive care unit and research facility.6 patients with an Injury Severity Score (ISS) of more than 25 points.Heparinized blood samples of severely injured patients and 12 healthy volunteers were incubated in vitro with 10 ng/ml GM-CSF, 10 ng/ml G-CSF or 10 ng/ml IFN-gamma for 6 h.Flow cytometry: HLA-DR expression on monocytes, B- and T-lymphocytes. ELISA: LPS-induced TNFalpha and IL-10 production.In all patients reduced cytokine production and HLA-DR expression on monocytes was established. After administration of GM-CSF and IFN-gamma it in vitro, the level of HLA-DR expression on monocytes and the it ex vivo TNFalpha-synthesis increased while only GM-CSF increased significantly IL-10-liberation after LPS-stimulation. However, only IFN-gamma had the capacity to enhance HLA-DR on B- and T-lymphocytes. G-CSF it in vitro had no significant effect on the measured parameter.These data suggest that GM-CSF and IFN-gamma may serve to support immune functions in severely injured patients. |
| Databáze: | OpenAIRE |
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