Effects of infliximab against carbon tetrachloride-induced intestinal injury via lipid peroxidation and apoptosis
| Autor: | Muhammed Kadri Çolakoğlu, Ahmet Pergel, Abdullah Özdemir, Gökhan Demiral, Adnan Yilmaz, Levent Tumkaya, Süleyman Kalcan, Tolga Mercantepe |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Male Health Toxicology and Mutagenesis CCL4 Apoptosis Pharmacology Toxicology medicine.disease_cause Protective Agents digestive system Lipid peroxidation Rats Sprague-Dawley 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Intestinal mucosa Malondialdehyde Intestine Small medicine Animals Carbon Tetrachloride Inhalation exposure business.industry Superoxide Dismutase digestive oral and skin physiology General Medicine Small intestine Infliximab Intestinal Diseases 030104 developmental biology medicine.anatomical_structure chemistry 030220 oncology & carcinogenesis Carbon tetrachloride Lipid Peroxidation business Oxidative stress |
| Zdroj: | Humanexperimental toxicology. 38(11) |
| ISSN: | 1477-0903 |
| Popis: | Carbon tetrachloride (CCL4) is often employed in the production of chlorofluorocarbons, petroleum refining, oil and rubber processing, and laboratory applications. Oral, subcutaneous, and inhalation exposure to CCL4 in animal studies have been shown to be capable of leading to various types of cancer (benign and malignant, liver, breast, and adrenal gland tumors). The present study also evaluated the protective role of infliximab (INF) against the deleterious effects of CCL4 on the intestinal system. Twenty-four male Sprague-Dawley rats were randomly assigned into three groups, control ( n = 8), CCL4 ( n = 8), and CCL4 + INF ( n = 8). The control group received 1 mL isotonic saline solution only via intraperitoneal (i.p.) injection. The CCL4 group received a single i.p. dose of 2 mL/kg CCL4. The CCL4 + INF group received a single i.p. dose of 7 mg/kg INF followed 24 h later by a single dose of 2 mL/kg CCL4. All rats were euthanized 2 days following drug administration. CCL4 group samples also exhibited diffuse loss of enterocytes, vascular congestion, neutrophil infiltration, an extension of the subepithelial space and significant epithelial lifting along the length of the villi with a few denuded villous tips. In addition, CCL4 treatment increased intestinal malondialdehyde (MDA) level and caspase-3 positivity. On the other hand, INF decreased MDA levels, caspase-3 positivity, and loss of villous. Our findings suggest that CCL4 appears to exert a highly deleterious effect on the intestinal mucosa. On the other hand, INF is effective in preventing this CCL4-induced intestinal injury by reducing oxidative stress and apoptosis. |
| Databáze: | OpenAIRE |
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