Results of a pilot study using self‐collected mid‐turbinate nasal swabs for detection of influenza virus infection among pregnant women
| Autor: | De-Kun Li, Roxana Odouli, Mark G. Thompson, Donna E. David, Jennifer K. Meece, Jeannette Ferber, Pat Shifflett, Allison L. Naleway, Alicia M. Fry, Sarah Spencer, Sam Bozeman |
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| Rok vydání: | 2015 |
| Předmět: |
Adult
Pulmonary and Respiratory Medicine medicine.medical_specialty Epidemiology Pilot Projects Nose Turbinates medicine.disease_cause Polymerase Chain Reaction Sensitivity and Specificity Ribonuclease P Virus Specimen Handling Pregnancy Internal medicine Influenza Human medicine Influenza A virus Humans Pregnancy Complications Infectious Respiratory system Acute respiratory illness nasal swab business.industry Public Health Environmental and Occupational Health Original Articles medicine.disease Confidence interval self-collection Influenza B virus Infectious Diseases medicine.anatomical_structure Specimen collection Nasal Swab Immunology Female influenza business |
| Zdroj: | Influenza and Other Respiratory Viruses |
| ISSN: | 1750-2659 1750-2640 |
| Popis: | Background We evaluated the feasibility of asking pregnant women to self-collect and ship respiratory specimens. Methods In a preliminary laboratory study, we compared the RT-PCR cycle threshold (CT) values of influenza A and B viruses incubated at 4 storage temperatures (from 4 to 35°C) for 6 time periods (8, 24, 48, 72, and 168 hours and 30 days), resulting in 24 conditions that were compared to an aliquot tested after standard freezing (−20°C) (baseline condition). In a subsequent pilot study, during January–February, 2014, we delivered respiratory specimen collection kits to 53 pregnant women with a medically attended acute respiratory illness using three delivery methods. Results CT values were stable after storage at temperatures |
| Databáze: | OpenAIRE |
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