Altered neutrophil immunophenotypes in childhood B‑cell precursor acute lymphoblastic leukemia
| Autor: | Daniel B. Aranha, Arissa Ikeda, Simone F. Maia, Bianca F. Baglioli, Daniela Ribeiro Ney Garcia, Marcelo Gerardin Poirot Land, Elen Oliveira, Maria Luiza Macedo Silva, Elaine Sobral da Costa, Alberto Orfao, Nathalia Lopez‑Duarte, Tomasz Szczepański, Lukasz Sedek, Juana Ciudad, Thiago S. Bacelar, Maria Cecília Menks Ribeiro, Indyara C. Machado, Lisandra A. C. Teixeira |
|---|---|
| Přispěvatelé: | Conselho Nacional de Desenvolvimento Científico e Tecnológico (Brasil), Fundação Carlos Chagas Filho de Amparo à Pesquisa do Estado do Rio de Janeiro, Ministerio de Economía y Competitividad (España), European Commission, Ministerio de Educación, Cultura y Deporte (España), Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (Brasil) |
| Rok vydání: | 2015 |
| Předmět: |
0301 basic medicine
Male multiparameter flow cytometry Neutrophils Lymphocyte CD33 residual hematopoiesis altered neutrophil immunophenotype Immunophenotyping 03 medical and health sciences 0302 clinical medicine Precursor B-Cell Lymphoblastic Leukemia-Lymphoma medicine Genetic predisposition Humans Child B cell childhood business.industry Altered neutrophil immunophenotype Residual hematopoiesis Childhood Multiparameter flow cytometry B-cell precursor acute lymphoblastic leukemia Haematopoiesis 030104 developmental biology medicine.anatomical_structure Oncology 030220 oncology & carcinogenesis Immunology Female Interleukin-3 receptor Bone marrow business Research Paper |
| Zdroj: | Oncotarget Digital.CSIC. Repositorio Institucional del CSIC instname |
| ISSN: | 1949-2553 |
| Popis: | An increasing number of evidences suggest a genetic predisposition in acute lymphoblastic leukemia (ALL) that might favor the occurrence of the driver genetic alterations. Such genetic background might also translate into phenotypic alterations of residual hematopoietic cells. Whether such phenotypic alterations are present in bone marrow (BM) cells from childhood B-cell precursor (BCP)-ALL remains to be investigated. Here we analyzed the immunophenotypic profile of BM and peripheral blood (PB) maturing/matured neutrophils from 118 children with BCP-ALL and their relationship with the features of the disease. Our results showed altered neutrophil phenotypes in most (77%) BCP-ALL cases. The most frequently altered marker was CD10 (53%), followed by CD33 (34%), CD13 (15%), CD15/CD65 (10%) and CD123 (7%). Of note, patients with altered neutrophil phenotypes had younger age (p = 0.03) and lower percentages of BM maturing neutrophils (p = 0.004) together with greater BM lymphocyte (p = 0.04), and mature B-cell (p = 0.03) counts. No significant association was found between an altered neutrophil phenotype and other disease features. These findings point out the potential existence of an altered residual hematopoiesis in most childhood BCP-ALL cases. This work was supported by the following grants: Bilateral Cooperation Program between Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPES (Brasília/Brazil) and Dirección General de Políticas Universitárias – Ministério de Educación, Cultura y Deportes - DPGU (Madrid/Spain) (311/15) and RD12/0036/0048 from RETICS, (Instituto de Salud Carlos III, Ministerio de Economia y Competitividad, Madrid, Spain and Fondos FEDER); Fundação de Amparo à Pesquisa do Estado do Rio de Janeiro of Brazil (E26/110.105/2014; E26/102.191/2013); Conselho Nacional de Desenvolvimento Científico e Tecnológico – CNPQ of Brazil (400194/2014–7). EO and DNRG were both supported by grants from CAPES (Brazil). NLD was partly supported by a grant from CNPQ (Brazil). TS and LS were supported by the internal grants from the Medical University of Silesia (Katowice, Poland). |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|