Microvilli elongate in response to hydrogen peroxide and to perturbations of intracellular calcium
| Autor: | G. E. Marshall, William R. Lee, J. G. Edwards, R. G. Sutcliffe, G. G. Reid |
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| Rok vydání: | 1997 |
| Předmět: |
Cell type
Xanthine Oxidase Thapsigargin Brush border Biology Calcium in biology Cell Line chemistry.chemical_compound Fluorescence microscope Animals Humans Hydrogen peroxide Pigment Epithelium of Eye Egtazic Acid Calcimycin Chelating Agents Fluorescent Dyes Organelles Ionophores Microvilli Cell Membrane Retinal Cell Biology Hydrogen Peroxide Cell biology Microscopy Electron chemistry Aminoquinolines Microscopy Electron Scanning Calcium Indicators and Reagents Intracellular Signal Transduction |
| Zdroj: | Experimental cell research. 236(1) |
| ISSN: | 0014-4827 |
| Popis: | Using scanning electron microscopy and fluorescence microscopy, we have found that apical microvilli of diverse cell types, including nonepithelial cells, elongate in culture in response to the oxidative stress of hydrogen peroxide. The microvilli induced in culture on retinal pigment epithelial cells display a 30-nm axial periodicity similar to that described for stable microvilli of intestinal brush border. Microvilli can also be induced to elongate by chelating intracellular Ca2+and by the Ca2+-uptake inhibitor thapsigargin. Thus a response of microvillar protrusion occurs widely and may be related to depletion of intracellular calcium stores. |
| Databáze: | OpenAIRE |
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