Analyzing terephthalate metabolites in human urine as biomarkers of exposure: Importance of selection of metabolites and deconjugation enzyme
| Autor: | Volker Harth, Ruthann A. Rudel, Anna-Maria Andersson, Jochen F. Mueller, Carl-Gustaf Bornehag, Amrit Kaur Sakhi, Cathrine Thomsen, Thomas Brüning, Russ Hauser, Holger M. Koch, Frederik Lessmann, Marike Kolossa-Gehring, Hanne Frederiksen, Joseph M. Braun, Shanna H. Swan |
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| Rok vydání: | 2018 |
| Předmět: |
chemistry.chemical_classification
Chromatography 010401 analytical chemistry Clinical Biochemistry Phthalic Acids Cell Biology General Medicine Urine 010501 environmental sciences Tandem mass spectrometry 01 natural sciences Biochemistry Article 0104 chemical sciences Analytical Chemistry Phthalic acid chemistry.chemical_compound Enzyme chemistry Tandem Mass Spectrometry Humans Biomarkers Chromatography High Pressure Liquid Selection (genetic algorithm) 0105 earth and related environmental sciences |
| Zdroj: | Journal of Chromatography B. :91-92 |
| ISSN: | 1570-0232 |
| DOI: | 10.1016/j.jchromb.2018.09.035 |
| Popis: | Owing to their toxicity, phthalate plasticizers are currently being replaced with terephthalates in many consumer products. Nevertheless, data on human exposure to and toxicity of terephthalates are still scarce. In this study, we developed a robust analytical method for the measurement of six terephthalate metabolites (TPhMs) in human urine through their successful separation from phthalate metabolites (PhMs). Target analytes were identified, using commercially available standards, and quantified with isotopically labeled internal standards (IS). The limit of quantification (LOQ) of TPhMs were in the range of 0.12 to 0.4 ng/mL, with the exception of 2.8 ng/mL for terephthalic acid (TPA) and 3.75 ng/mL for mono-(2-ethylhexyl) terephthalate (mEHTP), which were found in procedural blanks at notable levels. The method developed in this study showed excellent accuracy (recoveries: 86–117%) and precision (RSD: 0.6–12.2%) for TPhMs. The method was successfully applied for the analysis of 30 human urine samples collected from individuals with no known history of occupational exposure. The detection frequencies (df %) of TPhMs in urine ranged between 26.6 and 100%. This is one of the first studies that report a method for the analysis of emerging class of environmental chemicals in human specimens. |
| Databáze: | OpenAIRE |
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