Serial analysis of the effects of methimazole therapy on circulating B cell subsets in Graves' disease
| Autor: | Juana Ciudad, M T Mories, J. J. Corrales, A. López, A. Orfao |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
Adult
Male Thyroid Hormones medicine.medical_specialty Endocrinology Diabetes and Metabolism medicine.medical_treatment Graves' disease B-Lymphocyte Subsets CD5 Antigens Iodide Peroxidase Thyroglobulin Antibodies CD19 Endocrinology Antithyroid Agents In vivo Internal medicine medicine Humans Lymphocyte Count Prospective Studies B cell Methimazole biology Receptors IgE business.industry Antithyroid agent CD23 Antibodies Monoclonal Middle Aged Flow Cytometry medicine.disease Graves Disease medicine.anatomical_structure Fluorescent Antibody Technique Direct biology.protein Female Antibody CD5 business |
| Zdroj: | Journal of Endocrinology. 151:231-240 |
| ISSN: | 1479-6805 0022-0795 |
| DOI: | 10.1677/joe.0.1510231 |
| Popis: | The immunosuppressive effects of antithyroid drug therapy are well recognized; however, the cellular mechanisms underlying their action remain largely unknown. In the present paper we have prospectively analyzed the in vivo effects of methimazole treatment on a large number of circulating B cell subsets, involved in the effector phase of the immune response, in a group of 18 hyperthyroid patients with Graves' disease (GD). The patients were sequentially studied before (day 0) and 7, 14, 30, 90 and 180 days after methimazole therapy. The results were compared with both a group of 19 age- and sex-matched healthy controls and a group of 20 untreated/euthyroid GD patients in long-term remission. The combination of flow cytometry and three colour immunofluorescence revealed a clear increase (PPPPPin vivo, GD patients treated with methimazole exhibited an abnormal but rather stable pattern of B cell distribution, similar to that present in hyperthyroid untreated GD patients, except for the CD5+ and CD5 − B cell populations. Our findings suggest that in vivo methimazole therapy would not directly have an important influence on circulating B cell subsets. Journal of Endocrinology (1996) 151, 231–240 |
| Databáze: | OpenAIRE |
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