Evaluation of vaporized hydrogen peroxide sterilization on the in vitro efficacy of meropenem-impregnated polymethyl methacrylate beads
| Autor: | Myra E Durham, Johanna R. Elfenbein |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Staphylococcus aureus
medicine.drug_class Antibiotics In Vitro Techniques Agar plate chemistry.chemical_compound Column chromatography medicine Animals Polymethyl Methacrylate Horses Hydrogen peroxide Chromatography General Veterinary Chemistry Elution Sterilization Osteomyelitis Hydrogen Peroxide Meropenem General Medicine Staphylococcal Infections Sterilization (microbiology) equipment and supplies Antimicrobial Microspheres Anti-Bacterial Agents Horse Diseases Vaporized hydrogen peroxide |
| Zdroj: | American Journal of Veterinary Research. 80:45-50 |
| ISSN: | 0002-9645 |
| DOI: | 10.2460/ajvr.80.1.45 |
| Popis: | OBJECTIVE To evaluate the effects of vaporized hydrogen peroxide (VHP) sterilization on the in vitro antimicrobial efficacy of meropenem-impregnated polymethyl methacrylate (M-PMMA) beads. SAMPLE 6-mm-diameter polymethyl methacrylate beads that were or were not impregnated with meropenem. PROCEDURES Meropenem-free polymethyl methacrylate and M-PMMA beads were sterilized by use of an autoclave or VHP or remained unsterilized. To determine the antimicrobial efficacy of each bead-sterilization combination (treatment), Mueller-Hinton agar plates were inoculated with 1 of 6 common equine pathogens, and 1 bead from each treatment was applied to a sixth of each plate. The zone of bacterial inhibition for each treatment was measured after 24 hours. To estimate the duration of antimicrobial elution into a solid or liquid medium, 1 bead from each treatment was transferred every 24 hours to a new Staphylococcus aureus–inoculated agar plate or a tube with PBS solution, and an aliquot of the eluent from each tube was then applied to a paper disc on an S aureus–inoculated agar plate. All agar plates were incubated for 24 hours, and the zone of bacterial inhibition was measured for each treatment. RESULTS In vitro antimicrobial efficacy of M-PMMA beads was retained following VHP sterilization. The duration of antimicrobial elution in solid and liquid media did not differ significantly between unsterilized and VHP-sterilized M-PMMA beads. CONCLUSIONS AND CLINICAL RELEVANCE Results indicated that M-PMMA beads retained in vitro antimicrobial activity and eluted the drug for up to 2 weeks after VHP sterilization. |
| Databáze: | OpenAIRE |
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