Equine hydrallantois is associated with impaired angiogenesis in the placenta
| Autor: | Hossam El-Sheikh Ali, Peter Daels, Alan T. Loynachan, Kirsten E. Scoggin, Pouya Dini, Mariano Carossino, Barry A. Ball, Karen E. Wolfsdorf |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Polyhydramnios
Vascular Endothelial Growth Factor A Placenta Diseases Angiogenesis Placenta Andrology Allantois Pregnancy Interstitial fluid Edema medicine Animals Horses Vascular Endothelial Growth Factor Receptor-1 Neovascularization Pathologic Chemistry Obstetrics and Gynecology Transplacental Hypoxia (medical) Vascular Endothelial Growth Factor Receptor-2 Chorioallantoic membrane HIF1A medicine.anatomical_structure Reproductive Medicine Pregnancy Animal Female Horse Diseases medicine.symptom Microvascular Density Developmental Biology |
| Zdroj: | Placenta. 93:101-112 |
| ISSN: | 0143-4004 |
| DOI: | 10.1016/j.placenta.2020.03.001 |
| Popis: | Introduction Hydrallantois is the excessive accumulation of fluid in the allantoic cavities during the last trimester of pregnancy, leading to abdominal wall hernias, cardiovascular shock, abortion, and dystocia. It has been postulated that hydrallantois is associated with structural and/or functional changes in the chorioallantoic membrane. In the present study, we hypothesized that angiogenesis is impaired in the hydrallantoic placenta. Method Capillary density in the hydrallantoic placenta was evaluated in the chorioallantois via immunohistochemistry for Von Willebrand Factor. Moreover, the expression of angiogenic genes was compared between equine hydrallantois and age-matched, normal placentas. Results In the hydrallantoic samples, edema was the main pathological finding. The capillary density was significantly lower in the hydrallantoic samples than in normal placentas. The reduction in the number of vessels was associated with abnormal expression of a subset of angiogenic and hypoxia-associated genes including VEGF, VEGFR1, VEGFR2, ANGPT1, eNOS and HIF1A. We believe that the capillary density and the abnormal expression of angiogenic genes leads to tissue hypoxia (high expression of HIF1A) and edema. Finally, we identified a lower expression of genes associated with steroidogenic enzyme (CYP19A1) and estrogen receptor signaling (ESR2) in the hydrallantoic placenta. Discussion Based on the presented data, we believe that formation of edema is due to disrupted vascular development (low number of capillaries) and hypoxia in the hydrallantoic placenta. The edema leads to further hypoxia and consequently, causes an increase in vessel permeability which leads to a gradual increase in interstitial fluid accumulation, resulting in an insufficient transplacental exchange rate and accumulation of fluid in the allantoic cavity. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect