Melatonin Supplementation for the Cryopreservation of Canine Sperm
| Autor: | Kianne Silva Monteiro, Naiara Cristina Motta, Ana Cláudia Pereira Cardoso, Stefânia Priscilla de Souza, Luis David Solis Murgas |
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| Rok vydání: | 2022 |
| Předmět: | |
| Zdroj: | Biopreservation and Biobanking. |
| ISSN: | 1947-5543 1947-5535 |
| Popis: | Antioxidants can be used in sperm cryopreservation protocols to reduce oxidative stress that occurs due to the cryopreservation process. The aim of this study was to evaluate the effects of melatonin supplementation on quality and oxidative stress parameters in cryopreserved canine sperm. Eighteen sperm ejaculates were collected from 6 Frenchie Bulldog males (3 collections per male). Sperm motility parameters, membrane integrity, and sperm morphology were analyzed before the cryopreservation process. The extender used in cryopreservation was composed of Tris-egg yolk and ethylene glycol 5% was added as a cryoprotectant. The cryoprotective medium was supplemented with 1.0, 1.5, 2.0, 2.5, and 3.0 mM melatonin, and the control group (without melatonin). Post-thaw sperm was evaluated as described for fresh sperm and oxidative stress parameters (lipid peroxidation, catalase, and superoxide dismutase). Post-thaw sperm motility parameters, membrane integrity, sperm morphology, and oxidative stress parameters did not differ ( |
| Databáze: | OpenAIRE |
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