The Protective Effects of the Ethyl Acetate Part of Er MiaoSan on Adjuvant Arthritis Rats by Regulating the Function of Bone Marrow-Derived Dendritic Cells
| Autor: | Min Liu, Xiaoyi Jia, Zihua Xuan, Ning Wang, Meng li Liu, Jiemin Ding |
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| Rok vydání: | 2020 |
| Předmět: |
CD86
0303 health sciences CD40 Article Subject biology Chemistry medicine.medical_treatment Interleukin Spleen Pharmacology Proinflammatory cytokine Other systems of medicine 03 medical and health sciences 0302 clinical medicine medicine.anatomical_structure Cytokine Complementary and alternative medicine 030220 oncology & carcinogenesis medicine biology.protein Tumor necrosis factor alpha RZ201-999 CD80 030304 developmental biology |
| Zdroj: | Evidence-Based Complementary and Alternative Medicine, Vol 2020 (2020) |
| ISSN: | 1741-4288 1741-427X |
| Popis: | Aims. The aim of this study was to evaluate the protective effects of Er Miao San (EMS) and the regulative function of bone marrow-derived dendritic cells (BMDCs) on adjuvant arthritis (AA) in rats. Methods. The ethyl acetate part of EMS (3 g/kg, 1.5 g/kg, and 0.75 g/kg) was orally administered from day 15 after immunization to day 29. The polyarthritis index and paw swelling were measured, the ankle joint pathological changes were observed using hematoxylin-eosin (HE) staining, and the spleen and thymus index were determined. Moreover, T and B cell proliferation were determined using the CCK-8 assay. The expression of BMDC surface costimulatory molecules and inflammatory factors were determined using flow cytometry and ELISA kits, respectively. Results. Compared with the AA model rats, the ethyl acetate fraction of EMS obviously reduced paw swelling (from 1.0 to 0.7) and the polyarthritis index (from 12 to 9) P < 0.01 and improved the severity of histopathology P < 0.01 . The treatment using ethyl acetate fraction of EMS significantly reduced the spleen and thymus index P < 0.01 and inhibited T and B cell proliferation P < 0.01 . Moreover, EMS significantly modulated the expression of surface costimulatory molecules in BMDCs, including CD40, CD80, CD86, and major histocompatibility complex class II (MHC-II) P < 0.01 . The results also showed that the ethyl acetate part of EMS significant inhibited the levels of proinflammatory cytokines interleukin- (IL-) 23 tumor necrosis factor- (TNF-) α and inflammatory factor prostaglandin (PG) E2 in the supernatant of BMDCs. However, the level of anti-inflammatory cytokine IL-10 was significantly increased P < 0.01 . Conclusion. These results suggest that the ethyl acetate part of EMS has better protective effects on AA rats, probably by regulating the function of BMDCs and modulating the balance of cytokines. |
| Databáze: | OpenAIRE |
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