Freshly isolated mouse 4F7+ splenic dendritic cells process and present exogenous antigens to T cells
| Autor: | Gernot Gradehandt, Erwin Rüde, Anastassia Pavlidou, Mansour Mohamadzadeh, Jürgen Knop, Alexander Enk |
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| Rok vydání: | 1994 |
| Předmět: |
Male
Time Factors Ovalbumin T cell T-Lymphocytes Immunology Antigen presentation Antigen-Presenting Cells Cell Separation In Vitro Techniques Mice medicine Immunology and Allergy Cytotoxic T cell Animals Antigen-presenting cell Cells Cultured Mice Inbred BALB C CD40 biology Antigen processing Histocompatibility Antigens Class II Antibodies Monoclonal Dendritic cell Dendritic Cells Natural killer T cell Molecular biology Cell biology medicine.anatomical_structure Antigens Surface biology.protein Female Peptides Spleen |
| Zdroj: | European journal of immunology. 24(12) |
| ISSN: | 0014-2980 |
| Popis: | The antibody 4F7 was reported to recognize an epitope expressed on dendritic cells (DC) from various tissues. To study the ability of splenic 4F7+ dendritic cells to process antigen for presentation to CD4+ T cells, DC were enriched using a separation procedure avoiding overnight culture which could lead to an altered phenotype. These DC were used as antigen-presenting cells (APC) in stimulation cultures of major histocompatibility complex class II-restricted T cells. It was found that they induce antigen-dependent lymphokine production by T cells and therefore could present exogenous antigens. These processing takes place intracellularly, because fixation abrogates presentation to T cells. Moreover, antigen presentation needs intracellular processing within endo- or lysosomes as chloroquine-treatment prevents T cell activation. Titration of APC numbers revealed that contaminating APC most likely did not account for antigen-specific T cell activation by DC. No evidence was found for release of antigenic peptides or for partial antigen processing possibly done by cell surface located enzymes on DC. In conclusion, these results indicate that freshly enriched DC are able to process antigens similarly to other APC. |
| Databáze: | OpenAIRE |
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