Genomic Investigation of the Emergence of Invasive Multidrug-Resistant Salmonella enterica Serovar Dublin in Humans and Animals in Canada
| Autor: | Danielle Daignault, Chand S. Mangat, Sadjia Bekal, Jean Longtin, Rita Finley, Florence Doualla-Bell, E. Jane Parmley, Rebecca Irwin, Richard J. Reid-Smith, Michael R. Mulvey, Geneviève Côté, Brigitte Lefebvre, Brent P. Avery, Amrita Bharat |
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| Rok vydání: | 2019 |
| Předmět: |
Adult
Male Serotype Canada Salmonella Adolescent Virulence Biology medicine.disease_cause Epidemiology and Surveillance Microbiology Young Adult 03 medical and health sciences Plasmid Antibiotic resistance Drug Resistance Multiple Bacterial Genotype medicine Animals Humans Pharmacology (medical) Child Aged 030304 developmental biology Pharmacology Salmonella Infections Animal 0303 health sciences 030306 microbiology Infant Newborn Infant Salmonella enterica Genomics Middle Aged biology.organism_classification Multiple drug resistance Infectious Diseases Child Preschool Salmonella Infections Cattle Female Plasmids |
| Zdroj: | Antimicrobial Agents and Chemotherapy. 63 |
| ISSN: | 1098-6596 0066-4804 |
| DOI: | 10.1128/aac.00108-19 |
| Popis: | Salmonella enterica subsp. enterica serovar Dublin is a zoonotic pathogen that often leads to invasive bloodstream infections in humans that are multidrug resistant. Described here are the results of Canadian national surveillance of S. Dublin from 2003 to 2015 in humans and bovines, principally collected through the Canadian Integrated Program for Antibiotic Resistance Surveillance (CIPARS). An increase in human infections due to multidrug-resistant (MDR) S. Dublin was observed in 2010, many of which were bloodstream infections. Phylogenomic analysis of human and bovine isolates revealed a closely related network that differed by only 0 to 17 single nucleotide variants (SNVs), suggesting some potential transmission between humans and bovines. Phylogenomic comparison of global publicly available sequences of S. Dublin showed that Canadian isolates clustered closely with those from the United States. A high correlation between phenotypic and genotypic antimicrobial susceptibility was observed in Canadian isolates. IS26 replication was widespread among U.S. and Canadian isolates and caused the truncation and inactivation of the resistance genes strA and bla(TEM-1B). A hybrid virulence and MDR plasmid (pN13-01125) isolated from a Canadian S. Dublin isolate was searched against NCBI SRA data of bacteria. The pN13-01125 coding sequences were found in 13 Salmonella serovars, but S. Dublin appears to be a specific reservoir. In summary, we have observed the rise of invasive MDR S. Dublin in humans in Canada and found that they are closely related to bovine isolates and to American isolates in their mobile and chromosomal contents. |
| Databáze: | OpenAIRE |
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