Hippocampal enlargement in Bassoon-mutant mice is associated with enhanced neurogenesis, reduced apoptosis, and abnormal BDNF levels
| Autor: | Bettina Kracht, Mihai Constantin S. Ionescu, Frank Angenstein, Alexandra Heyden, Paolo Calabresi, Stefano Romorini, Constanze I. Seidenbecher, Veronica Ghiglieri, Eckart D. Gundelfinger |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2011 |
| Předmět: |
Doublecortin Domain Proteins
Animals Apoptosis Astrocytes Brain-Derived Neurotrophic Factor Cell Proliferation Cell Survival Dentate Gyrus Magnetic Resonance Imaging Mice Mice Mutant Strains Microtubule-Associated Proteins Nerve Tissue Proteins Neurogenesis Neurons Neuropeptides Organ Size metabolism [Neuropeptides] Hippocampus Hippocampal formation 0302 clinical medicine diagnostic imaging [Dentate Gyrus] genetics [Nerve Tissue Proteins] 0303 health sciences metabolism [Astrocytes] embryology [Dentate Gyrus] physiology [Neurogenesis] Mutant Strains medicine.anatomical_structure metabolism [Brain-Derived Neurotrophic Factor] metabolism [Neurons] genetics [Microtubule-Associated Proteins] genetics [Neuropeptides] medicine.medical_specialty Histology physiology [Cell Survival] doublecortin protein Biology Bsn protein mouse Presynapse Pathology and Forensic Medicine 03 medical and health sciences Internal medicine medicine ddc:610 030304 developmental biology Brain-derived neurotrophic factor metabolism [Nerve Tissue Proteins] genetics [Organ Size] Hippocampus proper Dentate gyrus metabolism [Microtubule-Associated Proteins] Cell Biology Radiography physiology [Apoptosis] Endocrinology nervous system Forebrain Neuroscience 030217 neurology & neurosurgery |
| Zdroj: | Cell & tissue research 346(1), 11-26 (2011). doi:10.1007/s00441-011-1233-3 Cell and Tissue Research; Vol 346 |
| Popis: | Mice mutant for the presynaptic protein Bassoon develop epileptic seizures and an altered pattern of neuronal activity that is accompanied by abnormal enlargement of several brain structures, with the strongest size increase in hippocampus and cortex. Using manganese-enhanced magnetic resonance imaging, an abnormal brain enlargement was found, which is first detected in the hippocampus 1 month after birth and amounts to an almost 40% size increase of this structure after 3 months. Stereological quantification of cell numbers revealed that enlargement of the dentate gyrus and the hippocampus proper is associated with larger numbers of principal neurons and of astrocytes. In search for the underlying mechanisms, an approximately 3-fold higher proportion of proliferation and survival of new-born cells in the dentate gyrus was found to go hand in hand with similarly larger numbers of doublecortin-positive cells and reduced numbers of apoptotic cells in the dentate gyrus and the hippocampus proper. Enlargement of the hippocampus and of other forebrain structures was accompanied by increased levels of brain-derived neurotrophic factor (BDNF). These data show that hippocampal overgrowth in Bassoon-mutant mice arises from a dysregulation of neurogenesis and apoptosis that might be associated with unbalanced BDNF levels. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|