Estrogen receptor beta dominates in baboon carotid after endothelial denudation injury
| Autor: | D. Du Toit, Juhana Frösen, Pekka Häyry, Etienne Myburgh, Einari Aavik |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
Male
medicine.medical_specialty Vascular smooth muscle medicine.drug_class Urinary Bladder Estrogen receptor 030204 cardiovascular system & hematology Biochemistry Muscle Smooth Vascular Catheterization 03 medical and health sciences 0302 clinical medicine Endocrinology Internal medicine biology.animal polycyclic compounds medicine Animals Estrogen Receptor beta RNA Messenger Receptor Molecular Biology Estrogen receptor beta 030304 developmental biology 0303 health sciences Messenger RNA biology Chemistry Immune Sera Uterus Estrogen Receptor alpha Immunohistochemistry 3. Good health Alternative Splicing Carotid Arteries Receptors Estrogen Estrogen biology.protein Female Endothelium Vascular Antibody hormones hormone substitutes and hormone antagonists Baboon Papio |
| Zdroj: | Molecular and cellular endocrinology. 182(1) |
| ISSN: | 0303-7207 |
| Popis: | Increasing evidence, mainly from rodents, suggests that the predominant estrogen receptor (ER) in arteries is the newly-described ERbeta. We have investigated the expression of the two ERs in baboon carotid artery before and after denudation injury. Prior to denudation, both full length receptors were detected in semiquantitative RT-PCR; in addition two ERalpha but no ERbeta splicing variants were found. After denudation, ERbeta mRNA increased five-fold and declined, whereas ERalpha mRNA expression remained low. Prior to and after denudation, two ERalpha-specific antibodies showed no reaction with the vessel wall. Instead, two affinity purified antisera to ERbeta demonstrated a weak but distinct reaction over vascular smooth muscle cells with predenudation specimens, escalating post-denudation and declining thereafter. The results suggest that selective targeting to ERbeta should be attempted when designing estrogen-based vasculoprotective drug therapies devoid of uterotrophic side effects. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect