The Transition from Gastric Intestinal Metaplasia to Gastric Cancer Involves POPDC1 and POPDC3 Downregulation
| Autor: | Lea Rath-Wolfson, Michal Herman-Edelstein, Sara Morgenstern, Rachel Gingold-Belfer, Gania Kessler-Icekson, Romy Zemel, Doron Boltin, Zohar Levi |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
QH301-705.5 Biology Catalysis Inorganic Chemistry 03 medical and health sciences 0302 clinical medicine Downregulation and upregulation medicine Physical and Theoretical Chemistry Biology (General) CDX2 Molecular Biology QD1-999 Spectroscopy POPDC1 (BVES) gastric cancer Organic Chemistry Mucin LGR5 Cancer Intestinal metaplasia General Medicine medicine.disease Computer Science Applications Chemistry 030104 developmental biology gastric intestinal metaplasia 030220 oncology & carcinogenesis Cancer research Immunohistochemistry POPDC3 Immunostaining |
| Zdroj: | International Journal of Molecular Sciences, Vol 22, Iss 5359, p 5359 (2021) International Journal of Molecular Sciences Volume 22 Issue 10 |
| ISSN: | 1661-6596 1422-0067 |
| Popis: | Intestinal metaplasia (IM) is an intermediate step in the progression from premalignant to malignant stages of gastric cancer (GC). The Popeye domain containing (POPDC) gene family encodes three transmembrane proteins, POPDC1, POPDC2, and POPDC3, initially described in muscles and later in epithelial and other cells, where they function in cell–cell interaction, and cell migration. POPDC1 and POPDC3 downregulation was described in several tumors, including colon and gastric cancers. We questioned whether IM-to-GC transition involves POPDC gene dysregulation. Gastric endoscopic biopsies of normal, IM, and GC patients were examined for expression levels of POPDC1-3 and several suggested IM biomarkers, using immunohistochemistry and qPCR. Immunostaining indicated lower POPDC1 and POPDC3 labeling in IM compared with normal tissues. Significantly lower POPDC1 and POPDC3 mRNA levels were measured in IM and GC biopsies and in GC-derived cell lines. The reduction in focal IM was smaller than in extensive IM that resembled GC tissues. POPDC1 and POPDC3 transcript levels were highly correlated with each other and inversely correlated with LGR5, OLFM4, CDX2, and several mucin transcripts. The association of POPDC1 and POPDC3 downregulation with IM-to-GC transition implicates a role in tumor suppression and highlights them as potential biomarkers for GC progression and prospective treatment targets. |
| Databáze: | OpenAIRE |
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