Use of proteoliposome as a vaccine against Trypanosoma cruzi in mice
| Autor: | Pietro Ciancaglini, F. Juarez Ramalho-Pinto, Fabiana R. Santos, Vanessa Migliaccio |
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| Rok vydání: | 2008 |
| Předmět: |
Male
Protozoan Vaccines Light Proteolipids Trypanosoma cruzi Detergents Antigens Protozoan Biochemistry Mice Trypanosomiasis parasitic diseases Animals Amastigote Molecular Biology Mice Inbred BALB C Antiparasitic Agents biology Chemistry Macrophages Intracellular parasite Organic Chemistry Cell Biology biology.organism_classification Lipids Molecular biology In vitro Blot Membrane protein Liposomes biology.protein Antibody Intracellular |
| Zdroj: | Chemistry and Physics of Lipids. 152:86-94 |
| ISSN: | 0009-3084 |
| DOI: | 10.1016/j.chemphyslip.2007.12.003 |
| Popis: | We have generated proteoliposomes carrying proteins of Trypanosoma cruzi for use as immunogens in BALB/c mice. T. cruzi trypomastigote and amastigote forms were sonicated and mixed with SDS, with 94% recovery of soluble proteins. To prepare proteoliposomes, we have used a protocol in which dipalmitoylphosphatidylcholine, dipalmitoyl-phosphatidylserine and cholesterol were incubated with the parasite proteins. BALB/c mice immunized with 20 μg were able to generate antibodies which, in Western blotting, reacted with the proteins of T. cruzi . We further investigated the ability of peritoneal cells from immunized mice to arrest the intracellular replication of trypomastigotes, in vitro . After 72 h of culture, the number of intracellular parasites in immunized macrophages decreased significantly, as compared to controls. Despite the fact that exposure of mice to T. cruzi proteins incorporated into proteoliposomes generate antibodies and activate macrophages, the immunized mice were not protected against T. cruzi intraperitoneal challenge. |
| Databáze: | OpenAIRE |
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