In vitro and in vivo testing of the hypocholesterolemic activity of ergosterol- and β-glucan-enriched extracts obtained from shiitake mushrooms (Lentinula edodes)
| Autor: | Diego Morales, Eva Tejedor-Calvo, Cristina Soler-Rivas, María Tabernero, Carlota Largo, Gonzalo Polo, Alejandro Ruiz-Rodríguez, Noelia Jurado-Chivato |
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| Přispěvatelé: | Comunidad de Madrid, Ministerio de Economía y Competitividad (España), Morales, Diego, Morales, Diego [0000-0002-4600-6673] |
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Male beta-Glucans Shiitake Mushrooms 03 medical and health sciences chemistry.chemical_compound Mice In vivo Ergosterol Animals Food science Fruiting Bodies Fungal Glucan chemistry.chemical_classification 030109 nutrition & dietetics biology Cholesterol Extraction (chemistry) Supercritical fluid extraction General Medicine biology.organism_classification Mice Inbred C57BL 030104 developmental biology Lentinula chemistry lipids (amino acids peptides and proteins) Erg Food Science |
| Zdroj: | Digital.CSIC. Repositorio Institucional del CSIC instname |
| ISSN: | 2042-650X |
| Popis: | Herein, a supercritical extraction plant (with a 6 L extraction cell) was successfully used to obtain ergosterol-enriched extracts from Lentinula edodes under the following conditions: a temperature of 40 °C, pressure of 225 bar, reaction time of 1–5 h, and the flow rate of 20 L h−1 for recirculated CO2. Moreover, ergosterol (ERG) and the SFE extract (SFE) with highest ergosterol concentration were microemulsified and submitted to in vitro digestion to study their ability to displace cholesterol from dietary mixed micelles (DMMs). ERG was also mixed with a β-glucan-enriched (33.5%) extract (BGE) obtained from L. edodes to investigate the synergies between them; the results indicated that all these extracts (including BGE without ERG) could reduce the cholesterol levels in the DMMs. However, when ERG and SFE were simultaneously administered to mice with a hypercholesterolemic diet, no significant differences in the serum cholesterol levels were detected as compared to the case of the control. However, when only BGE was administrated to another mice model previously induced with hypercholesterolemia, significant reduction in the cholesterol levels was noticed. This research was supported by the national R+D program from the Spanish Ministry of Science and Innovation (project AGL2014-26211-R) and the regional program from the Community of Madrid, Spain (S2013/ABI-2728). |
| Databáze: | OpenAIRE |
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