Molecular characteristics of Streptococcus agalactiae in a mother-baby prospective cohort study: Implication for vaccine development and insights into vertical transmission
| Autor: | Zhenjiang Yao, Guoming Wen, Shunming Li, Xiaohua Ye, Dan Guo, Xuelian Cao, Chuan'an Wu |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Serotype Vaccination Coverage Virulence Factors 030106 microbiology Virulence Biology medicine.disease_cause Serogroup Pilus law.invention Streptococcus agalactiae 03 medical and health sciences 0302 clinical medicine law Pregnancy Streptococcal Infections medicine Humans 030212 general & internal medicine Prospective Studies Prospective cohort study Phylogeny General Veterinary General Immunology and Microbiology Neonatal sepsis Streptococcal Vaccines Public Health Environmental and Occupational Health Infant Newborn medicine.disease Virology Infectious Disease Transmission Vertical Infectious Diseases Transmission (mechanics) Molecular Medicine Female Kappa |
| Zdroj: | Vaccine. 36(15) |
| ISSN: | 1873-2518 |
| Popis: | Background Streptococcus agalactiae (GBS) is a leading cause of neonatal sepsis and meningitis in many countries. This study aimed to determine the molecular characteristics of GBS colonized in mothers and their infants so as to provide implication for vaccine strategies and confirm vertical transmission. Methods A prospective cohort study was conducted to recruit 1815 mother-neonate pairs. All GBS isolates from pregnant women and her infants were tested for serotypes, multilocus sequence types and virulence genes. The relationship between multiple molecular characteristics of GBS isolates was tested by the correspondence analysis, and the agreement between mother-neonate paired data in molecular characteristics was analyzed using Kappa tests. Results The predominant serotypes were III, Ia and V, and the most prevalent sequence types (STs) were ST19, ST17, ST10, and ST12. All isolates carried at least one pilus island (PI). The most common combination of PIs was PI-2b alone, followed by PI-1+PI-2a and PI-2a alone, and the most prevalent alpha-like protein (alp) genes were rib, epsilon and alphaC. Moreover, a strong relationship was noted between STs, serotypes, alp genes and PIs, including ST17 associated with serotype-III/rib/PI-2b, ST19 with serotype-III/rib/PI-1+PI-2a, and ST485 with serotype-Ia/epsilon/PI-2b. The rate of GBS vertical transmission was 14.1%, and the kappa test revealed good agreement in multiple molecular characteristics among GBS-positive mother-neonate pairs. Notably, the switching of molecular characteristics was found during vertical transmission. Conclusions Our findings underscore the value of monitoring multiple molecular characteristics so as to provide implication for multivalent strategies and gain insights into GBS vertical transmission and vertical characteristic switching. |
| Databáze: | OpenAIRE |
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