Isolation and characterization of vibriophage vB_Vc_SrVc9: an effective agent in preventing Vibrio campbellii infections in brine shrimp nauplii (Artemia franciscana)
| Autor: | Francisco J. Magallón-Barajas, Lucy Gannon, Slawomir Michniewski, Andrew D. Millard, Carlos Omar Lomelí-Ortega, Eduardo Quiroz-Guzmán, Diana R. Barajas-Sandoval, Bernardo Veyrand-Quirós, Ana G. Reyes, Alexis de Jesús Martínez-Sández, Christian Harrison |
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| Rok vydání: | 2020 |
| Předmět: |
Phage therapy
Genes Viral medicine.medical_treatment Brine shrimp Genome Viral medicine.disease_cause Applied Microbiology and Biotechnology Microbiology Bacteriophage 03 medical and health sciences Vibrio Infections medicine Animals Bacteriophages Phage Therapy Vibrio campbellii 030304 developmental biology Vibrio 0303 health sciences biology 030306 microbiology Microbiota Pathogenic bacteria General Medicine biology.organism_classification Lytic cycle Artemia Biotechnology |
| Zdroj: | Journal of applied microbiologyReferences. 131(1) |
| ISSN: | 1365-2672 |
| Popis: | Aims This study describes the physicochemical and genomic characterization of phage vB_Vc_SrVc9 and its potential for phage therapy application against a pathogenic Vibrio campbellii strain. Methods and results A lytic phage vB_Vc_SrVc9 against Vibrio campbellii was isolated from shrimp farm sediment, and characterized physicochemical and genomically. The use of vB_Vc_SrVc9 phage increased the survival in Brine shrimp Artemia franciscana and reduced presumptive Vibrio campbellii to non-detectable numbers. Genomic analysis showed a genome with a single contig of 43.15 kb, with 49 predicted genes and no tRNAs, capable of recognizing and generating complete inhibition zones of three Vibrio species. Conclusions To our knowledge vB_Vc_SrVc9 is a lytic phage that could be used against Vibrio infections, reducing vibrio presence without any apparent impact over the natural microbiota at the family level in 28 libraries tested. Significance and impact of study vB_Vc_SrVC9 is a novel phage and ecofriendly alternative for therapeutic applications and biotechnological purposes because is stable at different environmental conditions, has a potential to eliminate several strains, and has a short latent period with a good burst size. Therefore, the use of phages, which are natural killers of bacteria, represents a promising strategy to reduce the mortality of farmed organisms caused by pathogenic bacteria. |
| Databáze: | OpenAIRE |
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