Automated analysis of foot-shock sensitivity and concurrent freezing behavior in mice
| Autor: | Darci M. Nielsen, Linda S. Crnic |
|---|---|
| Rok vydání: | 2002 |
| Předmět: |
Male
Electroshock medicine.medical_specialty Data collection Behavior Animal Computers General Neuroscience Audiology Foot shock Stimulus (physiology) Hindlimb Automated data Automation Mice Freezing behavior Reaction Time medicine Animals Humans Anxiety Visual observation medicine.symptom Psychology Simulation |
| Zdroj: | Journal of Neuroscience Methods. 115:199-209 |
| ISSN: | 0165-0270 |
| DOI: | 10.1016/s0165-0270(02)00020-1 |
| Popis: | Foot-shock is used in a variety of behavioral tasks either as a motivational stimulus, a way to characterize different rodents, or to test various pharmacological agents for their antinociceptive or analgesic effects. All these procedures need to assess foot-shock sensitivity either to rule out possible differences (when the shock is used as a motivational stimulus) or to use the differences to compare animals or treatments. In addition, many of the procedures that utilize foot-shock as a motivational stimulus evaluate freezing as an index of anxiety or fear. In the present study, data obtained by an automated computer system was compared with data obtained by human observers to validate the automated system for examining foot-shock sensitivity in mice. The different computer measures obtained for foot-shock sensitivity exhibited high correlations with human scoring at shock levels as low as 0.2 mA. The computer controlled analysis provided a non-subjective, quantifiable measurement of the foot-shock-induced response as well as freezing behavior. Automated data collection is an improvement over the methods of human visual observation in that the data collection is more standardized, efficient and consistent. |
| Databáze: | OpenAIRE |
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