Evidence for genetic control of changes in f-actin polymerization caused by pathogenic microorganisms: in vitro assessment using gingival fibroblasts from human twins
| Autor: | C. Walker, T. M. Hassell, E. L. Harris, A. Geinoz, P. Baehni, G. Gabbiani |
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| Rok vydání: | 1997 |
| Předmět: |
Adult
Male Adolescent Phalloidin Protein Conformation Gingiva Twins Connective tissue macromolecular substances Filamentous actin Microbiology chemistry.chemical_compound Bacterial Proteins medicine Humans Genetic Predisposition to Disease Cytoskeleton Fibroblast Child Porphyromonas gingivalis Cells Cultured Periodontal Diseases biology Cell-Free System Fusobacterium nucleatum Genetic Variation Fibroblasts Middle Aged biology.organism_classification Molecular biology In vitro Actins medicine.anatomical_structure chemistry Microscopy Fluorescence Periodontics Female Disease Susceptibility |
| Zdroj: | Journal of periodontal research. 32(1 Pt 2) |
| ISSN: | 0022-3484 |
| Popis: | Attachment to and migration upon a substratum, as well as other functions of connective tissue cells, are regulated mainly by cytoplasmic structural proteins, particularly filamentous actin (f-actin). Pathogenic microorganisms exert negative effects on cytoskeletal proteins. In the present study, normal gingival fibroblasts from 10 sets of human twins (6 fraternal, DZ; 4 identical, MZ) were exposed to soluble extracts from Porphyromonas gingivalis or Fusobacterium nucleatum, then f-actin was stained using FITC-labeled phalloidin. Cells were examined under fluorescence, and a computer-assisted image analyzer quantitated f-actin polymerization as fluorescence intensity on a per-cell basis. Intraclass correlation coefficients for f-actin in MZ/MZ vis-a-vis DZ/DZ paired cell cultures were determined to assess the possible heritability of responses to the microorganism preparations. F-actin labeling was significantly different between control cultures and those exposed to the extracts. Both F. nucleatum and P. gingivalis effected f-actin and fibroblast morphology. When the data were adjusted for gender and age effects, and for differences in control f-actin levels, fibroblasts from MZ twin pairs were moderately similar in both absolute and relative responses to bacterial challenges; cells from DZ twins showed little similarity when response was measured on the absolute scale, and moderate similarity using the relative scale. |
| Databáze: | OpenAIRE |
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