Sea Cucumber Lipid-Soluble Extra Fraction Prevents Ovalbumin-Induced Allergic Airway Inflammation
| Autor: | U-Cheol Jeong, Hak Sun Yu, Feng Jin, Jeong-Yeol Lee, Anisuzzaman, Shin Ae Kang, Da-In Lee, Seok-Joong Kang |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Ovalbumin Sea Cucumbers Anti-Inflammatory Agents Medicine (miscellaneous) Biology Pharmacology Mice 03 medical and health sciences Th2 Cells 0302 clinical medicine medicine Splenocyte Animals Humans Lung chemistry.chemical_classification Nutrition and Dietetics medicine.diagnostic_test Fatty Acids Fatty acid respiratory system Eosinophil biology.organism_classification Eicosapentaenoic acid Asthma Mice Inbred C57BL 030104 developmental biology Bronchoalveolar lavage medicine.anatomical_structure chemistry Immunology Apostichopus japonicus biology.protein Cytokines Th17 Cells Female 030211 gastroenterology & hepatology Cell activation Bronchoalveolar Lavage Fluid |
| Zdroj: | Journal of Medicinal Food. 21:21-29 |
| ISSN: | 1557-7600 1096-620X |
| DOI: | 10.1089/jmf.2017.3994 |
| Popis: | In a previous study, our research group demonstrated that sea cucumber (Apostichopus japonicus) extracts ameliorated allergic airway inflammation through CD4+CD25+Foxp3+ T (regulatory T; Treg) cell activation and recruitment to the lung. In this study, we aimed to determine which components of sea cucumber contribute to the amelioration of airway inflammation. We used n-hexane fractionation to separate sea cucumber into three phases (n-hexane, alcohol, and solid) and evaluated the ability of each phase to elevate Il10 expression in splenocytes and ameliorate symptoms in mice with ovalbumin (OVA)/alum-induced asthma. Splenocytes treated with the n-hexane phase showed a significant increase in Il10 expression. In the n-hexane phase, 47 fatty acids were identified. Individual fatty acids that comprised at least 5% of the total fatty acids were 16:0, 16:1n-7, 18:0, 18:1n-7, 20:4n-6, and 20:5n-3 (eicosapentaenoic acid). After administering the n-hexane phase to mice with OVA/alum-induced asthma, their asthma symptoms were ameliorated. Several immunomodulatory effects were observed in the n-hexane phase-pretreated group, compared with a vehicle control group. First, eosinophil infiltration and goblet cell hyperplasia were significantly reduced around the airways. Second, the concentrations of Th2-related cytokines (IL-4, IL-5, and IL-13) and Th17-related cytokines (IL-17) were significantly decreased in the spleen and bronchoalveolar lavage fluid (BALF). Finally, the concentrations of TGF-β and IL-10, which are associated with Treg cells, were significantly increased in the BALF and splenocyte culture medium. In conclusion, a fatty acid-rich fraction (n-hexane phase) of sea cucumber extract ameliorated allergic airway inflammation in a mouse model. |
| Databáze: | OpenAIRE |
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