Hyperbaric Oxygen Treatment Augments the Efficacy of a Losartan Regime in an Experimental Nephrotic Syndrome Model
| Autor: | Kayser Caglar, T. Alp Ikizler, Mahmut Ilker Yilmaz, Huseyin Aktug, Turgut Topal, Yusuf Oguz, Mujdat Yenicesu, Tayfun Eyileten, Abdulgaffar Vural, Alper Sonmez, Halil Yaman, Ahmet Korkmaz, Cengizhan Acikel, Sukru Oter, Ayper Kaya |
|---|---|
| Rok vydání: | 2006 |
| Předmět: |
Male
Nephrology medicine.medical_specialty Nephrotic Syndrome Physiology Inflammation Kidney Losartan Rats Sprague-Dawley Internal medicine Genetics medicine Animals Hyperbaric Oxygenation Proteinuria business.industry Drug Synergism Glomerulonephritis General Medicine medicine.disease Angiotensin II Rats Oxidative Stress Endocrinology Doxorubicin Anesthesia medicine.symptom business Nephrotic syndrome medicine.drug Kidney disease |
| Zdroj: | Nephron Experimental Nephrology. 104:e15-e22 |
| ISSN: | 1660-2129 |
| DOI: | 10.1159/000093260 |
| Popis: | Background/Aims: Proteinuria is associated with oxidant stress and inflammation. Hyperbaric oxygen (HBO) treatment has anti-inflammatory and anti-oxidant effects. The aim of the study was to investigate the benefits of HBO treatment on an experimental nephrotic syndrome model. Methods: 50 male Sprague-Dawley rats weighing 255 ± 39 g were housed. Forty rats were injected 6 mg/kg adriamycin into tail veins under anesthesia to induce nephrosis, while 10 rats were spared as sham control. After the stabilization of proteinuria at the sixth week, the rats were treated for 6 weeks by losartan (n = 10, 30 mg/kg/day), HBO (n = 10, 2.8 atmosphere absolute, 90 min/day), HBO + losartan (n = 10) and vehicle (n = 10). Protein carbonyl (PCO), superoxide dismutase (SOD) and glutathione peroxidase (GPx) were analyzed from tissue specimens. Biochemical markers were studied from venous samples and 24-hour urine was collected for proteinuria. The surviving animals at 12 weeks (vehicle group (n = 6), HBO (n = 6), losartan (n = 8), HBO + losartan (n = 10) were sacrificed. Glomerular sclerosis, tubulointerstitial and blood vessel changes were determined by semiquantitative scoring. Results: The PCO levels increased (p < 0.001), and the GPx and SOD levels decreased (p < 0.001 for both) in the nephrotic rats. In losartan and HBO groups GPx levels increased (p = 0.001, p = 0.002 respectively), but PCO and SOD levels did not change. The combination of HBO with losartan significantly increased the GPx and SOD levels (p = 0.001 for both) and decreased PCO levels (p = 0.005). HBO but not losartan significantly reduced proteinuria (p < 0.001). The combination of HBO and losartan reduced proteinuria better than the single losartan regime (p < 0.001). The effect of the combination was also noticed on the histological examination of the kidneys. The activities, appetites, weight gains, and improvement of edema were better in the HBO combined with losartan regime. Conclusions: These results indicate that the addition of HBO therapy to a conventional regime, angiotensin receptor blockers, has significant benefits in the management of proteinuria. Future clinical studies are needed to elucidate the role of HBO and other antioxidant strategies in the treatment of proteinuria. |
| Databáze: | OpenAIRE |
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