Marker-assisted elimination of drought-susceptible accessions in upland rice breeding
| Autor: | Claudio Brondani, Ricardo Diógenes Dias Silveira, Adriano Pereira de Castro, Gabriel Feresin Pantalião, Fern, a Martins Abreu, ro Gomide Neves, Rosana Pereira Vianello, Ariadna Faria Vieira, Ana Letycia Basso Garcia |
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| Přispěvatelé: | ARIADNA FARIA VIEIRA, UFG, GABRIEL FERESIN PANTALIAO, FERNANDA MARTINS ABREU, RICARDO DIAS SILVEIRA, ANA LETYCIA BASSO GARCIA, LEANDRO GOMIDE, ROSANA PEREIRA VIANELLO, CNPAF, ADRIANO PEREIRA DE CASTRO, CNPAF, CLAUDIO BRONDANI, CNPAF. |
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Candidate gene Genetic techniques and protocols Drought tolerance Melhoramento genético vegetal Genome-wide association study Single-nucleotide polymorphism Biology Upland rice Genotyping by sequencing Plant breeding 03 medical and health sciences Genotype Genetics SNP Allele Molecular Biology Resistência a seca Nucleotide sequences business.industry Sequence analysis food and beverages General Medicine Biotechnology Single nucleotide polymorphism 030104 developmental biology Arroz Rice business |
| Zdroj: | Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA-Alice) Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA |
| Popis: | Breeding for water-deficit tolerance is fundamental to guarantee the sustainability of upland rice production, mainly due to the possibility of an increased frequency of drought episodes due to climate change. This work aimed to identify single nucleotide polymorphism (SNP) markers, derived from RNA sequencing (RNA-Seq), genome-wide association study (GWAS) and candidate genes from Arabidopsis, with potential for use in marker-assisted selection (MAS) for drought tolerance. RNA-Seq and GWAS were efficient in identifying useful SNP markers from the data obtained from three years of field experiments for 175 upland rice accessions, which were sequenced using 32 genes by Capture-Seq. Three genes were equally able to generate SNP markers that discriminated 95% of the 20 most drought susceptible accessions in the joint analysis of the experiments. The elimination of the genotypes with the unfavourable SNP allele reduced the initial number of accessions to one third, and transferring this result in a breeding routine, would enable to conduct smaller experiments per target location, increasing the precision and reducing the cost of the drought phenotyping. Made available in DSpace on 2018-03-10T00:34:10Z (GMT). No. of bitstreams: 1 CNPAF2018gmr.pdf: 333924 bytes, checksum: 202e7a3c0bca406bccdf93cad82d91f8 (MD5) Previous issue date: 2018-03-08 |
| Databáze: | OpenAIRE |
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