Carbon as a Simple Support for Redox Biocatalysis in Continuous Flow
| Autor: | Sarah Ann Warren, Barnabas Poznansky, Lisa A. Thompson, Kylie A. Vincent, Holly A. Reeve |
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| Rok vydání: | 2019 |
| Předmět: |
Packed bed
Immobilized enzyme biocatalysis cofactor recycling 010405 organic chemistry Continuous operation Chemistry flow chemistry Communication Organic Chemistry packed bed Flow chemistry 010402 general chemistry Formate dehydrogenase 01 natural sciences Redox 0104 chemical sciences chemistry.chemical_compound Chemical engineering Biocatalysis Lactate dehydrogenase Physical and Theoretical Chemistry immobilized enzyme |
| Zdroj: | Organic Process Research & Development |
| ISSN: | 1083-6160 |
| Popis: | A continuous packed bed reactor for NADH-dependent biocatalysis using enzymes co-immobilized on a simple carbon support was optimized to 100% conversion in a residence time of 30 min. Conversion of pyruvate to lactate was achieved by co-immobilized lactate dehydrogenase and formate dehydrogenase, providing in situ cofactor recycling. Other metrics were also considered as optimization targets, such as low E factors between 2.5–11 and space-time yields of up to 22.9 g L–1 h–1. The long-term stability of the biocatalytic reactor was also demonstrated, with full conversion maintained over more than 30 h of continuous operation. |
| Databáze: | OpenAIRE |
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