Functional crosstalk in culture between macrophages and trigeminal sensory neurons of a mouse genetic model of migraine

Autor: Elsa Fabbretti, Asha Nair, Arn M. J. M. van den Maagdenberg, Andrea Nistri, Alessia Franceschini, Tanja Bele
Rok vydání: 2012
Předmět:
Patch-Clamp Techniques
Calcium Channels
L-Type
Sensory Receptor Cells
P2X3 receptor
Purinergic receptor
Pain
Neuroinflammation
ATP
Sensitization
Mice
Transgenic
Sensory system
Cell Communication
Biology
lcsh:RC321-571
Membrane Potentials
Mice
Cellular and Molecular Neuroscience
Trigeminal ganglion
Adenosine Triphosphate
Phagocytosis
Tubulin
Genetic model
medicine
Animals
lcsh:Neurosciences. Biological psychiatry. Neuropsychiatry
Cells
Cultured
Macrophages
General Neuroscience
lcsh:QP351-495
Calcium-Binding Proteins
Microfilament Proteins
Coculture Techniques
Ganglion
Mice
Inbred C57BL
Crosstalk (biology)
lcsh:Neurophysiology and neuropsychology
medicine.anatomical_structure
Animals
Newborn
Trigeminal Ganglion
Mutation
Settore BIO/14 - Farmacologia
Neuroscience
Receptors
Purinergic P2X3
Research Article
Zdroj: BMC Neuroscience
BMC Neuroscience, 13
BMC Neuroscience, Vol 13, Iss 1, p 143 (2012)
ISSN: 1471-2202
DOI: 10.1186/1471-2202-13-143
Popis: Background Enhanced activity of trigeminal ganglion neurons is thought to underlie neuronal sensitization facilitating the onset of chronic pain attacks, including migraine. Recurrent headache attacks might establish a chronic neuroinflammatory ganglion profile contributing to the hypersensitive phenotype. Since it is difficult to study this process in vivo, we investigated functional crosstalk between macrophages and sensory neurons in primary cultures from trigeminal sensory ganglia of wild-type (WT) or knock-in (KI) mice expressing the Cacna1a gene mutation (R192Q) found in familial hemiplegic migraine-type 1. After studying the number and morphology of resident macrophages in culture, the consequences of adding host macrophages on macrophage phagocytosis and membrane currents mediated by pain-transducing P2X3 receptors on sensory neurons were examined. Results KI ganglion cultures constitutively contained a larger number of active macrophages, although no difference in P2X3 receptor expression was found. Co-culturing WT or KI ganglia with host macrophages (active as much as resident cells) strongly stimulated single cell phagocytosis. The same protocol had no effect on P2X3 receptor expression in WT or KI co-cultures, but it largely enhanced WT neuron currents that grew to the high amplitude constitutively seen for KI neurons. No further potentiation of KI neuronal currents was observed. Conclusions Trigeminal ganglion cultures from a genetic mouse model of migraine showed basal macrophage activation together with enhanced neuronal currents mediated by P2X3 receptors. This phenotype could be replicated in WT cultures by adding host macrophages, indicating an important functional crosstalk between macrophages and sensory neurons.
Databáze: OpenAIRE
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