Multispectral optoacoustic imaging of dynamic redox correlation and pathophysiological progression utilizing upconversion nanoprobes
| Autor: | Ruochong Zhang, Zhimin Wang, Yuanjin Zheng, Yong Wang, Haolun Cheong, Mingyuan Gao, Bengang Xing, Xiangzhao Ai, Jun Lin |
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| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Science Multispectral image Wavelength channels Deep penetration Mice Nude General Physics and Astronomy Nanoprobe 02 engineering and technology Kidney Redox Theranostic Nanomedicine Article General Biochemistry Genetics and Molecular Biology Photoacoustic Techniques Mice 03 medical and health sciences Animals Humans lcsh:Science Lung Fluorescent Dyes Inflammation Mice Inbred BALB C Photons Spectroscopy Near-Infrared Multidisciplinary Chemistry Heart General Chemistry 021001 nanoscience & nanotechnology Photon upconversion Disease Models Animal RAW 264.7 Cells 030104 developmental biology Liver Molecular Probes Biophysics Nanoparticles Female lcsh:Q Spatiotemporal resolution Tomography X-Ray Computed 0210 nano-technology Oxidation-Reduction Biomarkers Spleen Optoacoustic imaging |
| Zdroj: | Nature Communications, Vol 10, Iss 1, Pp 1-11 (2019) Nature Communications |
| ISSN: | 2041-1723 |
| Popis: | Precise and differential profiling of the dynamic correlations and pathophysiological implications of multiplex biological mediators with deep penetration and highly programmed precision remain critical challenges in clinics. Here we present an innovative strategy by tailoring a powerful multispectral optoacoustic tomography (MSOT) technique with a photon-upconverting nanoprobe (UCN) for simultaneous visualization of diversely endogenous redox biomarkers with excellent spatiotemporal resolution in living conditions. Upon incorporating two specific radicals-sensitive NIR cyanine fluorophores onto UCNs surface, such nanoprobes can orthogonally respond to disparate oxidative and nitrosative stimulation, and generate spectrally opposite optoacoustic signal variations, which thus achieves compelling superiorities for reversed ratiometric tracking of multiple radicals under dual independent wavelength channels, and significantly, for precise validating of their complex dynamics and correlations with redox-mediated pathophysiological procession in vivo. Reactive oxygen (ROS) and nitrogen (RNS) species are involved in key physiological processes and their balance is altered in various human diseases. Here the authors develop near-infrared upconversion nanoprobes to screen ROS/RNS dynamics simultaneously by multispectral optoacoustic imaging in vivo. |
| Databáze: | OpenAIRE |
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