Ceftriaxone Attenuated Anxiety-Like Behavior and Enhanced Brain Glutamate Transport in Zebrafish Subjected to Alcohol Withdrawal
| Autor: | Jotele Fontana Agostini, Niuany Viel Mendes, Samira Leila Baldin, Henrique Teza Bernardo, Maria Cecília Manenti, Naithan Ludian Fernandes Costa, Karolyne de Pieri Pickler, Eduardo Pacheco Rico |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
animal structures Alcohol Drinking Thiobarbituric acid Glutamic Acid Pharmacology Anxiety medicine.disease_cause Biochemistry 03 medical and health sciences Cellular and Molecular Neuroscience chemistry.chemical_compound Glutamatergic 0302 clinical medicine Neuromodulation mental disorders medicine Animals Zebrafish Ethanol Ceftriaxone Glutamate receptor Brain General Medicine medicine.disease Substance Withdrawal Syndrome Oxidative Stress 030104 developmental biology medicine.anatomical_structure chemistry Alcohol withdrawal syndrome embryonic structures 030217 neurology & neurosurgery Oxidative stress medicine.drug |
| Zdroj: | Neurochemical research. 45(7) |
| ISSN: | 1573-6903 |
| Popis: | Chronic and/or excessive consumption of alcohol followed by reduced consumption or abstention can result in Alcohol Withdrawal Syndrome. A number of behavioral changes and neurological damage result from ethanol (EtOH) withdrawal. Ceftriaxone (Cef) modulates the activity of excitatory amino acid transporters by increasing their gene expression. Zebrafish are commonly used to study alcohol exposure. The aim of this study was to evaluate the influence of Cef (100 µM) on behavior patterns, glutamate transport activity, and oxidative stress in zebrafish brains subjected to EtOH (0.3% v/v) withdrawal. The exploratory tests using Novel tank showed that EtOH withdrawal promoted a decrease in the time spent and number of entries of in the bottom displaying an anxiety-like behavior. In contrast, treatment with Cef resulted in recovery of exploratory behavioral patterns. Ceftriaxone treatment resulted in increased glutamate uptake in zebrafish subjected to EtOH withdrawal. Furthermore, EtOH withdrawal increased reactive species, as determined using thiobarbituric acid and dichlorodihydrofluorescein assays. Treatment with Cef reversed these effects. Ceftriaxone promoted a significant reduction in brain sulfhydryl content in zebrafish subjected to EtOH withdrawal. Therefore, Cef treatment in conjunction with EtOH withdrawal induced anxiolytic-like effects due to possible neuromodulation of glutamatergic transporters, potentially through mitigation of oxidative stress. |
| Databáze: | OpenAIRE |
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