Effect of laser welding with human serum albumin on the expression of P-selectin on platelets
| Autor: | Alistar Phillips, Diane Felsen, Dix P. Poppas, Adam H Hamawy, Antonio Lauto, Bernardo Cuomo, Robert A. Soslow, Sandra J. Shin, Irena Kirman, Eli Heldman |
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| Rok vydání: | 1999 |
| Předmět: |
Blood Platelets
medicine.medical_specialty P-selectin Serum albumin Platelet Membrane Glycoproteins Dermatology In Vitro Techniques Flow cytometry Dogs In vivo Internal medicine medicine Animals Platelet Platelet activation Serum Albumin medicine.diagnostic_test biology Chemistry Albumin Flow Cytometry Platelet Activation Human serum albumin P-Selectin Carotid Arteries Endocrinology Immunology biology.protein Female Tissue Adhesives Surgery Laser Therapy medicine.drug |
| Zdroj: | Lasers in Surgery and Medicine. 25:438-444 |
| ISSN: | 1096-9101 0196-8092 |
| DOI: | 10.1002/(sici)1096-9101(1999)25:5<438::aid-lsm11>3.0.co;2-t |
| Popis: | Background and Objective Artery repair by means of laser energy induces activation of platelets with a risk of thrombosis and local inflammatory reactions. The aim of this study was to investigate the effect of human serum albumin, the most common solder in laser surgery, on platelet activation. Study Design/Materials and Methods Platelet activation was evaluated in canine blood by using two-color flow cytometry with a phycoerythrin-labeled antibody to a common platelet marker, glycoprotein IIb/IIIa and fluorescein isothiocyanate–labeled antibody to a platelet activation molecule, P-selectin. Human serum albumin was applied in vitro and in vivo, as a solder during laser reconstruction of canine arteries. Results In vitro, albumin significantly (P < 0.01) reduces the expression of P-selectin on platelets. This is most likely related to the blockage of P-selectin by albumin, which binds to the platelet surface, as confirmed by flow cytometry with fluorescein isothiocyanate–labeled albumin. In vivo, application of albumin solder tended to result in a lower percentage of P-selectin–expressing platelets in laser-repaired arteries compared to suture-repaired arteries. Conclusion Albumin decreases the percentage of P-selectin–expressing platelets in vitro. Further research may allow the platelet activation inhibiting properties of albumin to be further optimized in vivo. Lasers Surg. Med. 25:438–444, 1999. © 1999 Wiley-Liss, Inc. |
| Databáze: | OpenAIRE |
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