Discovery of Plasmodium modulators by genome-wide analysis of circulating hemocytes in Anopheles gambiae
| Autor: | Kristin Michel, Anastasios C. Koutsos, Sofia B. Pinto, Fotis C. Kafatos, Robert M. Waterhouse, Fabrizio Lombardo, Chandra Ramakrishnan, Chunju An, Krista McKay |
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| Jazyk: | angličtina |
| Rok vydání: | 2009 |
| Předmět: |
Genetic Markers
Anopheles gambiae/genetics Plasmodium Hemocytes Drosophila melanogaster/genetics Anopheles gambiae Genome Insect Antimicrobial peptides Aedes/genetics Microbiology Transcriptome Immune system Aedes cellular immunity innate immunity malaria mosquito Genome Insect/genetics Anopheles Hemolymph parasitic diseases Animals Plasmodium berghei ddc:576.5 RNA Messenger Multidisciplinary Innate immune system biology Gene Expression Profiling RNA Messenger/analysis fungi Gene Expression Regulation Developmental Biological Sciences biology.organism_classification Molecular biology Malaria Gene expression profiling Drosophila melanogaster Female Hemocytes/metabolism |
| Zdroj: | Proceedings of the National Academy of Sciences, Vol. 106, No 50 (2009) pp. 21270-5 |
| ISSN: | 0027-8424 |
| Popis: | Insect hemocytes mediate important cellular immune responses including phagocytosis and encapsulation and also secrete immune factors such as opsonins, melanization factors, and antimicrobial peptides. However, the molecular composition of these important immune cells has not been elucidated in depth, because of their scarcity in the circulating hemolymph, their adhesion to multiple tissues and the lack of primary culture methods to produce sufficient material for a genome-wide analysis. In this study, we report a genome-wide molecular characterization of circulating hemocytes collected from the hemolymph of adult female Anopheles gambiae mosquitoes—the major mosquito vector of human malaria in subSaharan Africa. Their molecular profile identified 1,485 transcripts with enriched expression in these cells, and many of these genes belong to innate immune gene families. This hemocyte-specific transcriptome is compared to those of Drosophila melanogaster and two other mosquitoes, Aedes aegypti and Armigeres subalbatus . We report the identification of two genes as ubiquitous hemocyte markers and several others as hemocyte subpopulation markers. We assess, via an RNAi screen, the roles in development of Plasmodium berghei of 63 genes expressed in hemocytes and provide a molecular comparison of the transcriptome of these cells during malaria infection. |
| Databáze: | OpenAIRE |
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