Relationship of colony-stimulating activity to apparent kill of human colony-forming cells by irradiation and hydroxyurea

Autor:	H E, Broxmeyer, P R, Galbraith, F L, Baker
Rok vydání:	1976
Předmět:	Cell Survival Immunology Bone Marrow Cells Cell Biology Hematology Biochemistry Radiation Effects Colony-Stimulating Factors Bone Marrow Gamma Rays Humans Hydroxyurea Cells Cultured Glycoproteins
Zdroj:	Blood. 47:403-411
ISSN:	1528-0020 0006-4971
DOI:	10.1182/blood.v47.3.403.bloodjournal473403
Popis:	Suspensions of human bone marrow cells were subjected to 137Cs irradiation in vitro and then cultured in semisolid agar medium. Cultures of irradiated cells were stimulated with colony-stimulating activity (CSA) of different potencies, and it was found that the amount of stimulation applied to cultures influenced the apparent kill of colony-forming cells (CFC). It was also found that the effects of irradiation on colony formation were not confined to CFC kill since medium conditioned by cells during irradiation exhibited stimulatory and inhibitory properties after treatment by 600 and 1000 rads, respectively. Studies in which irradiated cells were pretreated with hydroxyurea indicated that CFC in the DNA synthetic phase of the cell cycle were particularly sensitive to low doses of irradiation. The proliferative capacity of CFC surviving 1000 rads was undiminished as judged by their ability to form large colonies. Estimates of CFC kill by hydroxyurea were also affected by the level of CSA.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::84969b7d75a38f57decff91e25c051ae https://doi.org/10.1182/blood.v47.3.403.bloodjournal473403 Zobrazit plný text záznamu