Sequencing and assembly of a full-length infectious clone of grapevine virus B and its infectivity on herbaceous plants
| Autor: | Munir Mawassi, Henryk Czosnek, A. Fingstein, Y. Moskovitz, L. Bir, D. E. Goszczynski |
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| Rok vydání: | 2007 |
| Předmět: |
Genetics
Flexiviridae clone (Java method) Infectivity Grapevine virus B DNA Complementary biology Sequence analysis Molecular Sequence Data Nucleic acid sequence Sequence Homology General Medicine Genome Viral Sequence Analysis DNA biology.organism_classification Virology Complete sequence Complementary DNA Tobacco RNA Viral |
| Zdroj: | Archives of virology. 153(2) |
| ISSN: | 0304-8608 |
| Popis: | Grapevine virus B (GVB) has been found associated with corky bark-diseased vines. Although the sequence of a 7.6-kb cDNA clone from a GVB isolate from Italy has been described, striking differences in sequences between GVB isolates prompted us to construct an additional full-length GVB clone from the isolate 94/971 and to determine its complete sequence. The cDNA of GVB 94/971 shared a nucleotide sequence identity of only 77% with the GVB isolate from Italy. The cDNA of GVB 94/971 was infectious on Nicotiana plants as demonstrated by symptoms and by means of Northern blot, Western blot and electron microscopic analyses. |
| Databáze: | OpenAIRE |
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