Globin messenger RNA synthesis and processing during haemoglobin induction in Friend cells. II. Evidence for post-transcriptional control in clone 707
| Autor: | R. S. Gilmour, J. D. Windass, N.A. Affara, P.R. Harrison, J. Paul |
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| Rok vydání: | 1974 |
| Předmět: |
Cytoplasm
Five-prime cap Transcription Genetic Biology Tritium Cell Line Hemoglobins Mice Transcription (biology) Gene expression Animals Dimethyl Sulfoxide RNA Messenger RNA Neoplasm Globin Cell Nucleus Messenger RNA Base Sequence Nucleic Acid Hybridization RNA Templates Genetic Non-coding RNA Molecular biology Chromatin Clone Cells Polyribosomes Autoradiography Leukemia Erythroblastic Acute Small nuclear RNA Developmental Biology |
| Zdroj: | Cell Differentiation. 3:23-30 |
| ISSN: | 0045-6039 |
| Popis: | On exposure to 1.5–2% dimethylsulphoxide (DMSO) the M2 clone of the Friend erythroleukaemic cell is induced to form large amounts of haemoglobin. Complementary DNA (cDNA) synthesised on a template of mouse globin mRNA was used to measure the concentration of mRNA in RNA from different cell compartments. The globin mRNA content of polysomes increased 50- to 100-fold during induction whereas the globin mRNA content of nuclear RNA increased only 5- to 6-fold. The greatest increase, more than 8-fold, occurred in the largest nuclear RNA species. RNA made on chromatin templates was similarly tested. No globin mRNA sequences could be measured in this RNA from uninduced cells but significant amounts were found in chromatin-dependent RNA from induced cells. It is concluded that there is control of transcription of the globin gene in this cell and probably also control at a post-transcriptional level. |
| Databáze: | OpenAIRE |
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